Correlation of high-risk human papilloma viruses but not of herpes viruses or Chlamydia trachomatis with endometriosis lesions Peter Oppelt, M.D., M.B.A., Stefan P. Renner, M.D., Reiner Strick, Ph.D., Daniela Valletta, M.S., Grit Mehlhorn, M.D., Peter A. Fasching, M.D., Matthias W. Beckmann, M.D., Pamela L. Strissel, Ph.D. Fertility and Sterility Volume 93, Issue 6, Pages 1778-1786 (April 2010) DOI: 10.1016/j.fertnstert.2008.12.061 Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions
Figure 1 HPV-positive tissues using a PCR-based ELISA assay. Agarose gel electrophoresis demonstrating specific DNA fragments, which were positively detected in several tissues. Fragments include the beta-globin internal control gene and the 450-bp HPV L1 gene fragment (M = marker). Patient numbers and tissue types are indicated: E = endometrium; En = endometriosis; P = peritoneum. The L1-positive control DNA is included in the kit. All PCR fragments were also analyzed for ELISA for further HPV typing; for example, patient 14, a CxCa, was HPV16 positive; endometriosis from patient 8 was HPV medium risk; endometrium tissue from patient 6 was HPV16, whereas peritoneum from the same patient was negative for HPV (Tables 2 and 3). Fertility and Sterility 2010 93, 1778-1786DOI: (10.1016/j.fertnstert.2008.12.061) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions