P53 is responsible for the induction of apoptosis, developmental malformations and mortality of G1 and G2tert mutant zebrafish. p53 is responsible for.

Slides:

Advertisements

Similar presentations

Mitochondria localize to both the front and the rear of neutrophils.

Advertisements

Vegetal RNAs antagonise the dorsalising and BMP-inhibiting activity of Vrtn. Vegetal RNAs antagonise the dorsalising and BMP-inhibiting activity of Vrtn.

Strategy for F1 mutation carrier screening and identification of their mutations. Strategy for F1 mutation carrier screening and identification of their.

Obesity accompanied by increased triacylglyceride deposits in InsP3R mutants leads to starvation resistance. Obesity accompanied by increased triacylglyceride.

Figure 5 Chp1 downregulation in zebrafish leads to CaP-MN, cerebellar, and movement defects rescued with WT, but not mutant CHP1 mRNA Chp1 downregulation.

Tumor Necrosis Factor Signaling Mediates Resistance to Mycobacteria by Inhibiting Bacterial Growth and Macrophage Death Hilary Clay, Hannah E. Volkman,

nrde-3-dependent silencing of endogenous RNAi targets and transgenes.

Volume 18, Issue 1, Pages (July 2015)

Delayed Wound Healing in Immunodeficient TGF-β1 Knockout Mice

Characterization of cxcr3.2−/− embryos in unchallenged conditions.

The Chemokine SDF1a Coordinates Tissue Migration through the Spatially Restricted Activation of Cxcr7 and Cxcr4b Guillaume Valentin, Petra Haas, Darren.

Tumor Necrosis Factor Signaling Mediates Resistance to Mycobacteria by Inhibiting Bacterial Growth and Macrophage Death Hilary Clay, Hannah E. Volkman,

Effect of cxcr3.2 mutation on dissemination of local mycobacterial infection within 24 hpi. Effect of cxcr3.2 mutation on dissemination of local mycobacterial.

Tapetal mitochondria in wild-type (WT), drp3b, and elm1 mutants.

BTN1 mRNA level in response to extracellular pH determined by comparative RT2PCR. BTN1mRNA level in response to extracellular pH determined by comparative.

Fig. 2. Proportion of motile objects and track length

Fig. 3. Knockdown of cited3 results in increased cell death but it does not affect proliferation.Embryos that are injected with the control MO (A–C), cited3.

OA and related amines modulate motility and length of schistosomula.

Tomoregulin-1 improves cardiac pathological changes after pressure overload. Tomoregulin-1 improves cardiac pathological changes after pressure overload.

Fig. 4. Expression of miR-2 family mediates tissue growth of Hippo pathway.(A) Photomicrographs of adult wings of the indicated genotype. Expression of.

Embryonic corneal defects in E18.5 AP-2β NCC KO mutant embryos.

Inflammation is associated with increased basal-cell plasticity in the Nkx3.1 mutant prostate. Inflammation is associated with increased basal-cell plasticity.

Survival and telomere length of G2tert−/− zebrafish in a p53−/− background. Survival and telomere length of G2tert−/− zebrafish in a p53−/− background.

Patient and control ONS cells had very large differences in gene expression. Patient and control ONS cells had very large differences in gene expression.

Ectopic sprouting and proliferation in Rbpj veins.

eb1a-2 eb1b-3 double-mutant plants display skewed and shorter roots

The initial targeting of Sec61b mRNA to the ER is partially dependent on ribosomes and translation. The initial targeting of Sec61b mRNA to the ER is partially.

dex-1 expression in the seam cells is regulated by DAF-16.

Fgfr3 mutation is not the sole driver of tumorigenesis in the bladder.

Hipk induces hemocyte-derived melanotic tumors.

Effects of tumour size on response to treatment in the KPC model.

Expression data confirm and extend existing knowledge

Combined treatment with LY and sunitinib rescued hypervascularization.

tert mutant zebrafish can be bred for only one generation.

Effects of doxorubicin and epirubicin on proliferation and apoptosis in the hyperplastic livers of transgenic zebrafish larvae. Effects of doxorubicin.

Transduction with Cre-expressing adenoviruses induces cardiomyocyte apoptosis and shows that transgene insertion is not required. Transduction with Cre-expressing.

Kmt2b is required for SSC-to-progenitor transition.

Elevated expression of vegfaa in hemangiosarcoma.

GFP–Sec61b mRNA competes with t-ftz mRNA for the ribosome-binding sites on the ER. (A,B) COS7 cells were transfected with plasmid containing a test gene.

Table 1. Relative levels of tRNAMet and tRNASer in human pancreatic adenocarcinoma cell lines.Quantification of tRNAMet and tRNASer in pancreatic adenocarcinoma.

Simultaneous perturbations to shuttling result in defects in the Dl gradient and in the sna domain. Simultaneous perturbations to shuttling result in defects.

Fig. 5. Combination of SAHA and ML produces augmented suppression of cellular proliferation with impaired cell cycle progression, enhanced apoptotic.

Distribution and extent of expression.

Comparison ofMyc-induced zebrafish liver tumors with different stages of human HCC and seven mouse HCC models. Comparison ofMyc-induced zebrafish liver.

Characterization of zebrafish muscle wasting in krasG12V-induced HCC zebrafish. 4-month-old male adult krasG12V and WT zebrafish were treated with dox.

col22a1−/− mutant adults exhibit hemorrhages.

Apoptotic somatic cells in ovarian connective tissues.

Induction of liver tumors in TO(Myc) fish.

Fig. 1. PARN is differentially expressed in breast cancer versus non-cancerous tissue/cells. PARN is differentially expressed in breast cancer versus non-cancerous.

Effect of Dipy on the expression of chondrogenic markers.

Systemic infection in Drosophila larvae by septic injury with a fine tungsten needle. Systemic infection in Drosophila larvae by septic injury with a fine.

Drs expression profiles for OreR flies infected with the various C

(A) Western blot probing nuclear extract from wild-type (wt) and the newly generated ACF1 mutant (AcfC) embryos (0–16 h). (A) Western blot probing nuclear.

Gα12 is not required for hepatic cystogenosis induced by Pkd1 knockout

Fig. 4. Impaired induction of TH-response genes in trβ crispants

Fig. 3. Effects of Tec on IL-1β-induced apoptosis in chondrocytes.

Prophase nuclear movements in wild-type, rec8 and rec7 mutant cells.

Chd1 expression and CHD1 localization during mouse preimplantation development. Chd1 expression and CHD1 localization during mouse preimplantation development.

Overexpression of cyp7a1 in the liver ameliorates tumor-induced liver inflammation. Overexpression of cyp7a1 in the liver ameliorates tumor-induced liver.

Peroxisome speeds were slower in patient and control cells.

Fig. 4. Deletion of Baf60c in myocardium results in dilated chambers and impaired cardiac function. Deletion of Baf60c in myocardium results in dilated.

Fig. 6. Bj mutants show stereocilia patterning defects and biliary duct (BD) malformations. Bj mutants show stereocilia patterning defects and biliary.

Adult Acvr1;Trp53DCKO lenses form vascularized, tumor-like structures at their posterior poles. 4-month-old Trp53CKO and Acvr1;Trp53DCKO lenses were sectioned.

Ectopic expression of caveolin-1 in ZR75 human breast cancer cells downregulated survivin and decreased cell proliferation. Ectopic expression of caveolin-1.

BCL-3 regulates expression of stemness-associated Wnt targets.

Effect of siRNA transfection on colony formation by Renca cells.

Tsg protein stimulates endothelial cell migration, proliferation and collagen gel spheroid sprouting. Tsg protein stimulates endothelial cell migration,

AP2μ2 has an essential function in Wnt/β-Catenin signaling.

Mutations in the zebrafish hmgcs1 gene reveal a novel function for isoprenoids during red blood cell development by Jose A. Hernandez, Victoria L. Castro,

Fgfr1a, fgfr1b, and fgfr2 function redundantly to regulate pectoral fin development. fgfr1a, fgfr1b, and fgfr2 function redundantly to regulate pectoral.

Presentation transcript:

p53 is responsible for the induction of apoptosis, developmental malformations and mortality of G1 and G2tert mutant zebrafish. p53 is responsible for the induction of apoptosis, developmental malformations and mortality of G1 and G2tert mutant zebrafish. (A) Quantification of apoptotic cells based on TUNEL staining (see Materials and Methods). Significance was determined based on Student’s t-test (P<0.005). (B) Examples of embryos after TUNEL staining. (C) The mRNA levels of the p53 gene were determined by real-time RT-PCR in 72-hpf larvae, and muscle and testis tissues of adult zebrafish, in the indicated genotypes. Gene expression is normalized against rps11. Each bar represents the mean ± s.e.m. from 100 pooled animals for larvae and three individual fish for adult tissue and triplicate samples. (D) Survival of G2 tert−/− larvae microinjected at the one-cell stage with a morpholino against p53. (E) Kaplan-Meier representation of the survival of four genetic backgrounds. Post-hatching time is shown in weeks. The Log Rank test was used for statistical analysis. (F) Representation of total cell distribution from the four genotypes at the same age (11 months old) according to their telomere length. Medium fluorescence intensity (MFI) is indicated for each genetic background. The same trend was observed in the three independent experiments. (G) TUNEL assay in zebrafish testis sections from 8-month-old wild-type, tert mutant and double-mutant tert−/−; p53−/− zebrafish (400×). Arrowheads indicate apoptotic cells. Monique Anchelin et al. Dis. Model. Mech. 2013;6:1101-1112 © 2013. Published by The Company of Biologists Ltd