Analysis of Molecular Alterations in Left- and Right-Sided Colorectal Carcinomas Reveals Distinct Pathways of Carcinogenesis Tamotsu Sugai, Wataru Habano,

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Presentation transcript:

Analysis of Molecular Alterations in Left- and Right-Sided Colorectal Carcinomas Reveals Distinct Pathways of Carcinogenesis Tamotsu Sugai, Wataru Habano, Yu-Fei Jiao, Mitsunori Tsukahara, Yuichiro Takeda, Koki Otsuka, Shin-ichi Nakamura The Journal of Molecular Diagnostics Volume 8, Issue 2, Pages 193-201 (May 2006) DOI: 10.2353/jmoldx.2006.050052 Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 a: Relationship of mutations in p53, Ki-ras, and APC in LOH-high status tumors based on tumor location. b: Relationship of mutations in p53, Ki-ras, and APC in LOH-low status tumors based on tumor location. c: Relationship of mutations in p53, Ki-ras, and APC in CIMP-high status tumors based on tumor location. The Journal of Molecular Diagnostics 2006 8, 193-201DOI: (10.2353/jmoldx.2006.050052) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Frequencies of methylation of MINTI, MINT2, MINT31, MLH-1, MGMT, p14, p16, and RASSF1A promoters for left- and right-sided cancers. The Journal of Molecular Diagnostics 2006 8, 193-201DOI: (10.2353/jmoldx.2006.050052) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 3 A representative example of molecular alterations in left-sided cancer. b, d, f, h, and i: Multiple LOHs were seen (arrowhead). Although methylation at MINT 1 was found, MINT2, MINT31, p16, p14 (not shown), and RASSF1A promoters were not methylated. Furthermore, mutations of p53, Ki-ras, and APC genes were found (arrowhead). k: A GGC to GAC transition in Ki-ras codon 12 was found, resulting in a Gly to Asp substitution (sequence by reverse primer). l: A CTG to CAG transversion in exon 5 of p53 gene was observed. m: Finally, a CGA to TGA transition in codon 805 of APC gene was found, resulting in a stop codon. The three detectable mutated nucleotides in tumor DNA are indicated by arrows in the photograph (k, l, and m). The Journal of Molecular Diagnostics 2006 8, 193-201DOI: (10.2353/jmoldx.2006.050052) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 4 A representative example of molecular alterations in right-sided cancer. b, d, and f: Multiple LOHs were seen (arrowhead). Arrowheads indicate LOH. k, l, m, o, p, q, and r: In addition, multiple promoter regions were methylated (arrowhead). However, the p53 gene was not mutated (not shown). The Journal of Molecular Diagnostics 2006 8, 193-201DOI: (10.2353/jmoldx.2006.050052) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 5 The molecular profile of left- and right-sided colorectal cancers. The Journal of Molecular Diagnostics 2006 8, 193-201DOI: (10.2353/jmoldx.2006.050052) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions