In vivo tracking of PD-L1+NCMs in the microcirculation.

Slides:

Advertisements

Similar presentations

A prospectively validated csEN model accurately predicts dynamic changes of the maternal immune system over the course of pregnancy. A prospectively validated.

Advertisements

CsEN components reveal precisely timed cellular programs that characterize the dynamic changes of the peripheral immune system over the course of pregnancy.

Specific depletion of TFH and LCMV-specific CD4 T cells.

Comparison of the csEN algorithm to existing predictive methods and model reduction. Comparison of the csEN algorithm to existing predictive methods and.

Increased movement of GPR55 KO cells in association with epithelial cells. Increased movement of GPR55 KO cells in association with epithelial cells. (A)

The fast Brownian motility of DAG in B cell immunological synapse.

Demonstration of high-speed and precise actuation.

The functional and structural basis of phospholipid recognition by the PG90 TCR. The functional and structural basis of phospholipid recognition by the.

Three different types of transfer functions with a codomain of [0,1].

Self-sensing of actuator position.

Workspace comparison of Delta robots.

Analysis of Pentamer interfaces.

Ex vivo testing of the soft robotic devices.

Visual explanation of the interaction terms.

Visual explanation of the interaction terms.

Protection from autoimmunity is not due to the expansion of Treg subsets. Protection from autoimmunity is not due to the expansion of Treg subsets. (A.

RBC microswimmers for active cargo delivery.

The foldable robotic arm with seven modules assembled in series.

Comparison of repertoire distributions to baseline.

Fig. 7 Correlation of NHP and human ISGs.

Blood Tfr cells show expression of follicular and regulatory markers.

Blood Tfr cells are indicators of ongoing humoral activity.

Ag governs CTL behavior at islets but is not required for their accumulation in large numbers. Ag governs CTL behavior at islets but is not required for.

NCMs regulate T cell survival in TLOs via PD-L1.

PD-L1 selectively marks circulating NCMs.

PD-L1 expression is maintained on NCMs under inflammatory conditions and PD-L1+NCMs are found in TLOs. PD-L1 expression is maintained on NCMs under inflammatory.

Quasi-static and dynamic trajectories.

Crystal structures of RSV G-antibody complexes.

Cell viability tests. Cell viability tests. SEM images of (A) MC3T3-E1 cells and (B) MSCs on days 1, 3, and 5 of culture. (C) Survival rates of MC3T3-E1.

Experimental characterization of the milliDelta’s quasi-static workspace (yellow) compared with the theoretical workspace (blue) generated by the kinematic.

Experimental results for tremor reduction.

Fig. 3 Comparison between predicted change in DT since the LIG and observed sea-level highstands. Comparison between predicted change in DT since the LIG.

Fig. 2 Box plots of water use with lateral lengths.

Fig. 3 Oil, gas, and FP water variations with time.

Self-sensing of actuator position.

2D motility characterization and external magnetic steering of RBC microswimmers. 2D motility characterization and external magnetic steering of RBC microswimmers.

Origin of the asymmetry and determination of the critical angle

Sequence conservation within antigenic sites γ1 and γ2, atomic interactions within the RSV G CCD, and model of RSV G glycoprotein. Sequence conservation.

Active deformation of an RBC cargo propelled and pushed by a single bacterium through a 2-μm gap. Active deformation of an RBC cargo propelled and pushed.

Ligands eluted from hpMR1+EC and hpMR1+MS contain both shared and unique ions. Ligands eluted from hpMR1+EC and hpMR1+MS contain both shared and unique.

Affinities of the SV9 specific bs-868Z11-CD3 bsTCR with different MHC monomers and peptide ligands. Affinities of the SV9 specific bs-868Z11-CD3 bsTCR.

Fig. 2 Full-frame images recording the violation of a Bell inequality in four images. Full-frame images recording the violation of a Bell inequality in.

Fig. 1 Concept of the livestock transition in China between 1980 and Concept of the livestock transition in China between 1980 and The left-

BMS blocks functional responses in primary immune cells driven by IFNα

Fig. 1 Bioinspired design of AAD for promoting wound contraction.

CsEN components reveal precisely timed cellular programs that characterize the dynamic changes of the peripheral immune system over the course of pregnancy.

Fig. 5 Conceptual diagram of the impact of acid deposition on calcium cycling and subsequent plant water use. Conceptual diagram of the impact of acid.

Emergence of resonances and prediction of distinct network responses

S-Affs colocalize with SUMO in mammalian cells.

TRPML1 is required for fast DC migration, chemotaxis, and arrival at the LNs. TRPML1 is required for fast DC migration, chemotaxis, and arrival at the.

The prototype and working mechanism.

Galloping-like gait with the design of a two-legged robot.

Fig. 3 ET dynamics on the control and treatment watersheds during the pretreatment and treatment periods. ET dynamics on the control and treatment watersheds.

Fig. 5 Wave speed analysis.

Human Tfr cells do not express CD25.

Calcium release through TRPML1 controls myosin IIA retrograde flow at the rear of LPS-DCs. Calcium release through TRPML1 controls myosin IIA retrograde.

Fig. 2 Folding motions of the TCO with strain-softening behavior.

Fig. 5. Vascularization of human liver seed grafts.

Fig. 3 Coastal accretion rates.

Relationships between species richness and temperature or latitude

Discrimination accuracy across conditions.

Multiplexed four- and eight-channel devices for rapid processing

Fig. 3 Characterization of SGN responses to optogenetic stimulation.

Logarithmic fitness trajectory emerges from hopping between MSs

Cartoon model for the increased p-HLA display engendered by peptide splicing. Cartoon model for the increased p-HLA display engendered by peptide splicing.

Fig. 4 Effects of individual picosecond and microsecond pulses.

Fig. 2 Analysis of 1PCTCO2 experiments (JJA seasonal means).

Fig. 2 Imaging blood vessel before and after closure.

Comparison of children’s behavior between the three conditions.

Fig. 2 Time series of secularization versus GDP per capita, from four illustrative countries, over the 20th century. Time series of secularization versus.

Presentation transcript:

In vivo tracking of PD-L1+NCMs in the microcirculation. In vivo tracking of PD-L1+NCMs in the microcirculation. (A) Representative 3D time series showing PD-L1+ NCMs (red) crawling in the vasculature of the cremaster muscle. Tracks are shown in white. Vessels were reconstructed on the basis of intrinsic autofluorescent signals (see also movie S1). Arrows show flow direction. Yellow inset is shown in detail in (C). Scale bars, 30 μm. (B) 3D track plot of PD-L1+ NCMs from a single 10-min recording showing flow-independent trajectories in all directions. (C) Details from the yellow inset in (A), showing representative cell tracks color-coded according to their length, duration, average speed, and straightness (confinement ratio, C.R.), as depicted. (D to F) Analysis of cell motility. (D) Distribution of PD-L1+ NCM confinement ratio and (E) comparison between measured (red line, best fitted curve; see Materials and Methods) and random (green dashed line, predicted by linear regression fitted on the earliest time intervals) mean cell displacement over time. Values are shown as means ± 95% confidence interval (red dotted lines). (F) Average speed. Lines represent means. All data refer to 195 cells pooled from three independent experiments. Mariaelvy Bianchini et al. Sci. Immunol. 2019;4:eaar3054 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works