Sara Paveglio, PhD, MS, Erin Bennett, MS, Kelly L. Hawley, PhD, Adam P

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Presentation transcript:

FcεRI cross-linking reduces cord blood dendritic cell responsiveness to LPS Sara Paveglio, PhD, MS, Erin Bennett, MS, Kelly L. Hawley, PhD, Adam P. Matson, MD, MS Journal of Allergy and Clinical Immunology Volume 139, Issue 6, Pages 1992-1994.e3 (June 2017) DOI: 10.1016/j.jaci.2016.11.012 Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 A, Flow cytometry of CB mDCs demonstrating FcεRI expression and surface versus intracellular IgE. B, CB serum IgE levels versus total IgE bound by CB mDCs. C and D, Levels of FcεRI expression and total IgE compared between CB basophils and mDCs. E and F, Levels of TNF-α within CB mDCs after stimulation of CB leukocytes with LPS ± anti-FcεRI. G, Cytokine bead array from supernatants collected from purified CB mDCs stimulated with LPS ± anti-FcεRI (n = 6). MFI, Mean fluorescence intensity; NS, not significant. *P ≤ .05. Journal of Allergy and Clinical Immunology 2017 139, 1992-1994.e3DOI: (10.1016/j.jaci.2016.11.012) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Histamine reduces LPS-induced production of TNF-α by CB mDCs. A and B, Levels of TNF-α within CB mDCs following stimulation of CB leukocytes with LPS ± histamine. CB samples that contained high percentages of basophils loaded with IgE (C) were more likely to have reduced percentages of mDCs staining positive for TNF-α. Journal of Allergy and Clinical Immunology 2017 139, 1992-1994.e3DOI: (10.1016/j.jaci.2016.11.012) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E1 FcεRI stimulation does not change the baseline expression of CD83 or alter LPS-induced upregulation of CD83 on CB mDCs. Mixed CB leukocytes were stimulated with an anti-FcεRI antibody and/or LPS, and CB mDCs were analyzed by flow cytometry for surface CD83 expression. Data shown are the MFIs, with error bars indicating the SEM, for 5 CB samples (A), and a representative histogram from 1 CB sample (B). MFI, Mean fluorescence intensity; NS, not significant. **P ≤ .01. Journal of Allergy and Clinical Immunology 2017 139, 1992-1994.e3DOI: (10.1016/j.jaci.2016.11.012) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions