Fig. 5. The bulk of Cep135 localizes distantly from Sas-6 and STIL

Slides:

Advertisements

Similar presentations

Scanning electron microscopy analysis of EGK-I to -V chick embryos.

Advertisements

GM-CSF–producing γδT cells are involved in the development of EAM

Fig. 3 CSF1 is expressed in human melanoma.

KIN-3 depletion leads to increased levels of centrosomal ZYG-1

Fig. 5. Prip silencing enhances the co-localization of GABARAP with insulin vesicles and β-tubulin.Co-localization of GABARAP (green) with insulin (red)

Fig. 1. Overview of the nervous system of the adult S. roscoffensis.

Fig. 3. Hts and Dlg are in a complex at the postsynaptic membrane of larval NMJs.(A–B″) PLA with HtsM and Dlg antibodies (green) performed on third instar.

Fig. 1. Representative images of the four cell lines using fluorescence microscopy. Representative images of the four cell lines using fluorescence microscopy.

Fig. 4. smc3 regulates the expression of cx43 in regenerating fins.

Fig. 2. Morphological changes of cultured adherent fibroblastic cells after OA treatment related to actin microfilament reorganization.(A) Cells observed.

Fig. 7. Motion adaptation increases time-dependent response modulations (TDRM) relatively to the average cell response.TDRM normalized to the value obtained.

Fig. 2. Proportion of motile objects and track length

Fig. 6. Cross-section of the stomach wall and spiral intestine of the embryo, stained with PAS. (A) Surface of the stomach wall (SW) and ingested material.

Fig. 7. Knockdown of Meis1 abolishes CR4. 2-GFP expression

Fig. 2. Mapping of the interaction domain on coilin for association with the dyskerin complex. Mapping of the interaction domain on coilin for association.

Embryonic corneal defects in E18.5 AP-2β NCC KO mutant embryos.

Inflammation is associated with increased basal-cell plasticity in the Nkx3.1 mutant prostate. Inflammation is associated with increased basal-cell plasticity.

Fig. 1. Mitochondrial internalization in cardiomyocytes.

Fig. 4. Increased adipocyte differentiation in Cbx7-KO ES cells

Fig. 1. Morphological and growth characterization of hBMCs and hPDCs

Fig. 2. Transfection and clonal selection of rat pluripotent stem cells to generate stable transgenic lines. Transfection and clonal selection of rat pluripotent.

Fig. 4. The model of malate metabolism in fruit cells under different K level conditions. The model of malate metabolism in fruit cells under different.

Fig. 2. Salivary glands from RasV12-expressing larvae produce MMP1, release tissue fragments into the hemolymph and express apoptotic markers.Salivary.

Fig. 1. Pigmentation and melanophore counts of rainbow trout parr and smolt caudal fins.Pigmentation of (A) parr and (B) smolt. Pigmentation and melanophore.

Fig. 1. γ-Tubulin localizes in close proximity to centriole walls in interphase but within an extended PCM meshwork in mitosis.U2OS cells were fixed and.

Fig. 2. DDR1 over-expression enhances collagen fibril reorganization

Fig. 1. Ovarian cancer spheroids can bud from a monolayer

Dany is required for nuclear shape distortion around chromosome territories. Dany is required for nuclear shape distortion around chromosome territories.

Fig. 8. Knockdown of Meis1 reduces the expression of Foxn4 and Lim1+2

Fig. 6. LR phenotype of plastid translation-defective mutants with/without Spec. LR phenotype of plastid translation-defective mutants with/without Spec.

Formation of papilloma lesions in the UroIICre+Fgfr3+/K644EK-RasG12D/+ model. Formation of papilloma lesions in theUroIICre+Fgfr3+/K644EK-RasG12D/+model.

Fcp1 CTD phosphatase affects a broad range of transcripts in the +N and -N media. Fcp1 CTD phosphatase affects a broad range of transcripts in the +N and.

Fig. 4. Non-autonomous rescue of puc expression in DME cells

Fig. 2. Ex vivo inducible knockout of PDCD2 in ESCs results in loss of S phase entry and increased p53.(A) Growth curve of inducible knockout and WT ESCs.

Fig. 6. Comparison of Plk4 with Sas-6 localization

Fig. 6. Localization of Sma during growth and migration

Fig. 2. Morphological analysis of acentriolar mitotic spindles

Fig. 6. Effect of SAHA and ML on histone acetylation, BAX, and p21CDKN1A expression.PANC-1 and BxPC-3 cells were incubated for 48 hours with 5 µM.

Fig. 7. Lhx1-RNAi reduces the eye size

Fig. 2. Centrosomal proteins display distinct localizations and radial distances from centriole walls.U2OS cells were fixed and stained with the indicated.

Fig. 3. Rnd proteins induce stronger responses in subconfluent endothelial cells.HUVECs were transfected with Rnd1, Rnd2, Rnd3 or GFP-encoding plasmids.

Fig. 1. Aboveground biomass of Caragana and herbaceous plants, and proportional abundance of Caragana, under different grazing management treatments. Aboveground.

Fig. 7. Representative images of control (Cas9+GFP) and Cas9+gRNA+GFP co-injected embryos on day 4 of culture, showing nuclear-imported GFP (green) and.

BMP signalling is dispensable for early gastruloid patterning.

Fig. 8. The morphology of the ventral nerve cord in Ror-Myc overexpressing embryos is normal. The morphology of the ventral nerve cord in Ror-Myc overexpressing.

Fig. 1. Polarized F-actin cables in the Xenopus neural plate.

Effect of the plastid translation inhibitor Spec on LR development

Fig. 2. Immunostaining for ClC-5 in isolated hog gastric mucosa

Crim1 colocalizes with integrin and activates the integrin-FAK-ERK signaling pathway. Crim1 colocalizes with integrin and activates the integrin-FAK-ERK.

Distribution of replicating rDNA in the WT during S phase.

Depleting CK2 restores centrosomal ZYG-1 levels in zyg-1(it25) embryos

Fig. 3. Enhanced EGFR signaling in Rhbdf2P159L/P159L mice.

Single MTs can be resolved in subsections of SBF-SEM image stacks.

Fig. 2. iPSCs produce functional osteoblasts.

E4-ORF1 disrupts proper TJ localization of ZO-1 and ZO-2 in MDCK cells

Fig. 2. Acetylation stiffens primary cilia.

Fig. 5. Testis defects in STK35 KO mice.

Fig. 1. Rnd2 and Rnd3 induce stress fibres whereas Rnd1 reduces stress fibres in endothelial cells.(A) Rnd mRNAs are expressed in HUVECs. Total RNA was.

Fig. 6. RhoB is required for Rnd3-induced stress fibre formation

Fig. 3. MO-mediated smc3 knockdown results in reduced regenerate length, segment length and cell proliferation. MO-mediated smc3 knockdown results in reduced.

mip120 null egg chambers have a condensed nurse cell DNA phenotype

Depletion of COPI protein inhibits cis to trans cisternal maturation.

Fig. 5. Co-expression analyses of disease mutations in YFP-RPGRIP1α1 with wild-type RFP-RPGR1–19 or RFP-RPGRORF15 in COS7 cells.YFP-RPGRIP1α1 with disease-associated.

Table 1. Measurement of ring diameters of proteins localizing in ring-like patterns around centrioles.Consideration of the size of IgG (about 8 nm) raises.

Association of NM-HA and NM-GFP with SGs is transient.

WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates TfR. WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates.

Fig. 7. Rho family GTPase signaling is required for cyst formation in Ptp4E Ptp10D mutants.(A–C) Expression of DN Rho1 and Rac1 mutants in wild-type (w1118)

Fig. 7. Analysis of dFMRP kinetics in dFMRP granules by FRAP

Fig. 8. Expression of other genomic-clustered Chrn subunits in the mesodiencephalon.(A) Schematic representation illustrating the assembly of the Chrnb4,

Dynamic and polarized recruitment of ERC1a near protrusive sites during migration. Dynamic and polarized recruitment of ERC1a near protrusive sites during.

Presentation transcript:

Fig. 5. The bulk of Cep135 localizes distantly from Sas-6 and STIL Fig. 5. The bulk of Cep135 localizes distantly from Sas-6 and STIL.U2OS cells were stained with the indicated antibodies. The bulk of Cep135 localizes distantly from Sas-6 and STIL.U2OS cells were stained with the indicated antibodies. Representative 3D-SIM and wide-field (WF) images are shown. Scale bars 0.5 µm or 1 µm (overview image). (A) Localization of Sas-6, Cep135 and STIL in cells in S/G2 phase (before centrosome separation, upper panel) or late G2/M phase (after centrosome separation, lower panel). Wide-field images of the red (Cep135) and far-red (CP110) channels of the same centrosome were acquired to determine the orientation of centrioles (schematics illustrate centriole orientations and overview panel shows centrosome separation). (B) Brightness/contrast-enhanced image to indicate co-localization of weak Cep135 signal with Sas-6 (arrowheads). Katharina F. Sonnen et al. Biology Open 2012;1:965-976 © 2012. Published by The Company of Biologists Ltd