Fig. 3. The checkpoint proteins Mad2 and BubR1 remain associated with the kinetochores of unaligned chromosomes in cenp-metaΔ mutant cells entering anaphase.(A–C)

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Fig. 3. The checkpoint proteins Mad2 and BubR1 remain associated with the kinetochores of unaligned chromosomes in cenp-metaΔ mutant cells entering anaphase.(A–C) Selected frames from a WT (A) or two different cenp-metaΔ mutant cells (B,C) marked with GFP-Mad2 showing three mitotic stages (Prometaphase, Anaphase, Late anaphase). The checkpoint proteins Mad2 and BubR1 remain associated with the kinetochores of unaligned chromosomes in cenp-metaΔ mutant cells entering anaphase.(A–C) Selected frames from a WT (A) or two different cenp-metaΔ mutant cells (B,C) marked with GFP-Mad2 showing three mitotic stages (Prometaphase, Anaphase, Late anaphase). See the corresponding supplementary material Movies 4, 5, 6. Mad2 streaming during prometaphase is not affected in cenp-metaΔ mutant cells (B,C). While Mad2 signal is totally gone by the time the WT cell enters anaphase (A), it is still visible on kinetochores (arrowhead) of unaligned chromosomes at the poles and lasts until late anaphase in cenp-metaΔ mutant cells (B,C). (D–F) Selected frames from a WT (D) or two different cenp-metaΔ mutant cells (E,F) marked with GFP-Rod (green) and RFP-BubR1 (red). Note that GFP-Rod is used to monitor the anaphase onset since BubR1 signals are gone at this stage. BubR1 signal is no longer visible at metaphase (Met) in the WT cell (D) whereas it persists on unaligned kinetochores (arrowhead) seen in cenp-metaΔ mutant cells (E,F) entering anaphase (Ana). See also supplementary material Movies 7, 8, 9. Scale bars: 5 µm. Thomas Rubin et al. Biology Open 2014;bio.20148490 © 2014. Published by The Company of Biologists Ltd