(A) Deduced amino acid sequence of the D2SV cDNA cloned from the mouse heart RNA. The novel 20 amino acid C-terminus (CT) is underlined. (A) Deduced amino.

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(A) Deduced amino acid sequence of the D2SV cDNA cloned from the mouse heart RNA. The novel 20 amino acid C-terminus (CT) is underlined. (A) Deduced amino acid sequence of the D2SV cDNA cloned from the mouse heart RNA. The novel 20 amino acid C-terminus (CT) is underlined. The + signs above the CT indicate the conserved amino acid residues. (B) Comparison of functional domains of cyclin D1, D2 and D2SV. Binding sites for the members of retinoblastoma protein family (Rb), p21, CDK4, Dmp1 and estrogen receptor (ER) as well as the phosphorylation site of GSK-3β at Thr286 are indicated. Numbers indicate amino acid residues. Sequence homologies between different domains of D2SV and members of the cyclin D family (cycD1, D2 and D3) are also indicated. (C) Western blot analysis of HEK293 cells transfected with an empty pcDNA3.1 vector (Con) and pcDNA3.1-D2SV construct (D2SV) using polyclonal antibodies raised against the C-terminus of D2SV protein. Arrows indicate high molecular mass D2SV immunoreactive bands. Qian Sun et al. J Cell Sci 2009;122:1563-1573 © The Company of Biologists Limited 2009