Western blot analysis of histone H1.X.

Slides:

Advertisements

Similar presentations

A new method to address cross-reactivity of antibodies

Advertisements

1&3: hGH-sc(Fc)2 2&4: hGH-Fc anti-Fc anti-hGH

Invest. Ophthalmol. Vis. Sci ;44(9): doi: /iovs Figure Legend:

Effects of Growth Factors and Basement Membrane Proteins on the Phenotype of U- 373 MG Glioblastoma Cells as Determined by the Expression of Intermediate.

Interaction of SRP19 with nuclear transport receptors.

2D PAGE/Western blotting analysis for the identification of possible post-translation modification of Hsp27 from the spinal cord tissue of the SCI rat.

Fast protein LC IMAC purification of cytosolic phosphoproteins

Hours after rec PR-Set7 release Ladder kDa 15 PR-Set7 Coomassie

The scaffold protein APPL1 facilitates HDAC3 regulation of AKT

Endogenous SRP19 is localized to the nucleus and the nucleolus.

Insulin regulates the expression and intra-membrane proteolysis of the recombinant HA-pSREBP-1c-Flag protein in McA-RH7777 cells Insulin regulates the.

Production of recombinant KRBV NS1 and antiserum reactivity.

Analysis of whole cell lysates by Western blotting and 2D gel electrophoresis.A, cells overexpressing GFP fusion proteins and control cells were cultured.

2-D gel images visualized by Coomassie Brilliant Blue staining representing total proteins extracted from HCT-8 under apoptotic conditions in 2 mm Gln.

Western blotting analysis of purified cytoplasmic membranes.

Rita Das, Zhaolan Zhou, Robin Reed Molecular Cell

Titration of antisera against the amount of extract loaded on Western blots. Titration of antisera against the amount of extract loaded on Western blots.

Fig. 2. Sera IgE reactivity patterns and the frequency of reactivity to raw and cooked shrimp extracts by Western blot. (A, C) All sera were diluted at.

The histidine-rich loop regulates accessibility of the active site and RDEL motif in vivo. The histidine-rich loop regulates accessibility of the active.

PARP cleavage is detected in TA cells, but not in KSC

TP53 western blot of primary cultured tail cells from both wild-type (WT) and Tp53Δ11/Δ11 (−/−) rats. TP53 western blot of primary cultured tail cells.

Cross-reactivity of anti-Drosophila-synapsin with cockroach synapsin.

Caspases are activated earlier in young TA (yTA) than in KSC

(A) Deduced amino acid sequence of the D2SV cDNA cloned from the mouse heart RNA. The novel 20 amino acid C-terminus (CT) is underlined. (A) Deduced amino.

Characterisation of eSRS21 protein using an anti-eSRS21 antibody.

The expression of histone H1. X::GFP in C

SDS-PAGE (A,E,I) and immunoblot analysis (B–D, F–H, J–L) of SEC elution profiles of crystallins from D. mawsoni (A–D), T. obesus (E–H) and B. taurus (I–L).

Fig. 7. Ror-GFP binds to Myc-tagged Wnts and Wnt receptors.

H1. X depletion by RNA interference and colocalization of H1

C. elegans phenotypes created by H1. X RNA interference

High-affinity mutants of β3 integrin fail to stimulate RhoA activity and fibronectin fibrillogenesis. High-affinity mutants of β3 integrin fail to stimulate.

Interaction of periostin with ECM molecules.

IRS-1 and -2 expression in four mesangial cell lines from different D-NOD (A and B) and ND-NOD (C and D) mice. IRS-1 and -2 expression in four mesangial.

Cdc2 expression in cultured Schwann cells.

Silver-stained gel showing the major tyrosine-phosphorylated proteins in podocytes. Silver-stained gel showing the major tyrosine-phosphorylated proteins.

Effect of the siRNA-mediated knockdown of endogenous MARCH8 on the expression levels of MARCH8 substrates, TfR and CD98, in HepG2 cells. Effect of the.

Kinetics of GFP secretion from CHO cells.

Human sperm cells express CD44

A: Representative Western blot showing a band migrating at ∼90 kDa corresponding to neprilysin protein. A: Representative Western blot showing a band migrating.

Kinetics of BDNF-induced Erk, Akt and PLCγ activation in the presence of 15 mM NaCl or 15 mM KCl. Representative western blots (A) and quantitative plots.

Client-induced retrieval of ERp44.

Expression of IP3R subtypes in HEK cells.

LINT-25 and LAP2α interact in vitro.

Vps36 interacts with Smo in the absence of Hh

Subnuclear localization of HA-tagged EBNA1 protein.

The dynamics of Akt activation in cultured human keratinocytes.

GOLPH3 interacts with COG complex proteins.

Effect of overexpression of Gcs1p on mitochondrial morphology.

Increased electrophoretic mobility of Cx43 in the ischemic heart involves dephosphorylation at serine residues 325, 328 and/or 330. Increased electrophoretic.

4E-BP is present in an 80 kDa complex in unfertilized eggs.

Western blotting for GluR1.

Recognition of naturally processed HTLV-1 Tax protein derived from HAM patient's PBMC. A, HTLV-1 Tax protein content was evaluated by Western blot analysis.

Key functional sites of SPINDLIN1 could be phosphorylated by Aurora-A.

Western blot to show that the rabbit antihuman sclerostin antibody used in this study (Abcam, UK) recognises equine sclerostin. Western blot to show that.

Fig. 2. Western blot analysis of purified and enriched OMVs derived from O. tsutsugamushi. (A) Blots were revealed with FS15 monoclonal antibody against.

Western blot assay of an acute MIF-positive serum showing reactivity with the 135- and 115-kDa specific protein antigens of Rickettsia conorii and Rickettsia.

Western blot analysis of plasma proteins from two-dimensional gel electrophoresis. Western blot analysis of plasma proteins from two-dimensional gel electrophoresis.

Met is expressed in Her2-overexpressing cell lines and Her2 (+) breast tumors. Met is expressed in Her2-overexpressing cell lines and Her2 (+) breast tumors.

2D-PAGE gels showing differentially expressed proteins in Panc-1 cells transfected with siNR4A1 and functional classification of the proteins. 2D-PAGE.

Characterization of monoclonal antibody 4

Figure 2. Histoimmunoprecipitation and antigen identification

DNA-binding and double-strand break formation by Vpr.

Induction of PARP cleavage (A) and activation of caspases (B) after treatment with a combination of TRAIL and cisplatin. Induction of PARP cleavage (A)

Fig. 5 Dcr-2 directly binds to Toll 3′UTR.

Depletion of murine Dnmt proteins after 5-Aza-CdR treatment.

SDS-PAGE and Coomassie Blue Staining of Protein Samples after Various Purification Steps of Both Rice and Barley ADPG Hydrolytic NPPs.Lane numbering of.

SDS-PAGE and Immunoblot Analyses of Cucurbita PP1 and PP2 in Phloem Exudates of 13-Day-Old Intergeneric Grafts of Cucumiss ativus on Cucurbita maxima.(A)

Effect of LPS on ERK1 and ERK2 activation in human monocytes.

Expression of MtrE by wild-type and mtr120 mutant strains.

Clade 2 ERFs Differentially Bind and Activate a PMT2 Promoter

Presentation transcript:

Western blot analysis of histone H1.X. Western blot analysis of histone H1.X. (A,B) shows Coomassie-bluestained SDS page gels obtained from a total lysate of C. elegans, the recombinant C. elegans proteins lamin, intermediate filament proteins A1 and B1 and recombinant H1.X. (C) shows a corresponding blot stained with the αH1.X-101 antibody and (D) shows a corresponding blot stained with the αH1.X-11 antibody. No antibody is reactive with intermediate filament proteins. Both antibodies recognize H1.X as a band with an apparent molecular mass of 31 kDa. αH1.X-11 is crossreactive with C. elegans lamin and a further not identified protein. Monika A. Jedrusik et al. J Cell Sci 2002;115:2881-2891 © The Company of Biologists Limited 2002