Fig. 2. The localisation of integrins β1 and α6 in the hES cells grown in a colony and as a single-cell culture. hES cells were harvested manually as small.

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Fig. 2. The localisation of integrins β1 and α6 in the hES cells grown in a colony and as a single-cell culture. hES cells were harvested manually as small colony clumps or detached with EDTA and grown in the presence or absence of Y-27632 (single-cell culture) for 24 h. The localisation of integrinsβ1 and α6 in the hES cells grown in a colony and as a single-cell culture. hES cells were harvested manually as small colony clumps or detached with EDTA and grown in the presence or absence of Y-27632 (single-cell culture) for 24 h. (A) Integrin β1 was labelled with P5D2 antibodies (recognising total integrin β1) and with 12G10 (associating with active integrin β1) and imaged by confocal microscopy using an oil-immersed objective (60×). Integrin α6 was detected with a respective antibody. (B) The effect of the blocking antibody P5D2 on the localisation of integrin β1. The hES cells were detached with EDTA in the presence of Y-27632 and re-seeded in the presence of the integrin β1-neutralising antibody P5D2 or with an irrelevant control antibody for 24 h. The localisation of integrins β1 and α6 was analysed with fluorescence microscopy using a 40× objective. Scale bars: A, 0.01 mm; B, 0.05 mm. Ade Kallas-Kivi et al. Biology Open 2018;7:bio034355 © 2018. Published by The Company of Biologists Ltd