Effects of SYCE2 expression on DSB repair.

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Effects of SYCE2 expression on DSB repair. Effects of SYCE2 expression on DSB repair. (A) Schematic representation of the in vivo DNA-ligation assay that was performed to investigate the effects of SYCE2 expression on the efficiency of NHEJ. (B) Western blot analysis of RAD51 (upper panel) in two independent RPE-FLAG-SYCP3 clones. CDK2 expression (lower panel) is shown as a loading control. Cells were harvested before irradiation or at 2 h after irradiation. (C) Cell cycle distribution of the two independent RPE-FLAG-SYCE2 clones and mock cells analyzed by fluorescent activated cell sorting (FACS) in the steady state (left) and at 2 h after 8-Gy irradiation (right). Columns and bars represent the mean of three independent experiments and SD, respectively. (D, E) Percentages of cells containing more than five large foci of the BRCA1, which promotes HR. Columns and bars represent the mean of three independent experiments and SD, respectively. A total of 100 cells were examined for each cell line. Cells were stained before irradiation or at 2 h after 8-Gy irradiation. Noriko Hosoya et al. LSA 2018;1:e201800021 © 2018 Hosoya et al.