Fig. 7. ML-60218-mediated suppression of SAHA-induced up-regulation of tRNA as a potential mechanism that contributes to the enhanced anti-cancer actions.

Slides:

Advertisements

Similar presentations

Glioma-derived soluble factor(s) enhance IL-10 signaling.

Advertisements

A. B. BxPC-3 PANC h h h h h 100 *** * 72 h Cell viability (%) 80 *** *** Cell viability (%) 80 *** *** 60 *** ***

Histone Deacetylase Inhibitors Sensitize Human Non-small Cell Lung Cancer Cells to Ionizing Radiation Through Acetyl p53-Mediated c-myc Down-Regulation

Fig. 2. Mechanism of PD-L1 down-regulation in NOD HSPCs.

Cytotoxic therapy induces macrophage recruitment, as well as CSF1 and IL-34 mRNA expression. Cytotoxic therapy induces macrophage recruitment, as well.

Collagen-dependent phosphorylation of eIF4E in PDAC cells.

Mechanism of piperlongumine induced Sp downregulation.

Acetylation of histone 3 in MEF2C and GATA4 loci is enhanced in differentiating 22q11.2DS hiPSCs. Acetylation of histone 3 in MEF2C and GATA4 loci is enhanced.

Fig. 5. Effect of MPO on surface elasticity of human platelets

Fig. 7. Motion adaptation increases time-dependent response modulations (TDRM) relatively to the average cell response.TDRM normalized to the value obtained.

Fig. 2. Proportion of motile objects and track length

Fig. 4. A primary screen based on scrape closure

Fig. 2. p85β regulates cell adhesion

Fig. 3. Inactivation of the Wnt/β-catenin signaling pathway inhibited cell proliferation and induced apoptosis in A549 and SPC-A-1 cells. Inactivation.

Fig. 4. The model of malate metabolism in fruit cells under different K level conditions. The model of malate metabolism in fruit cells under different.

Fig. 1. Coilin interaction with the telomerase complex protein dyskerin is mediated by hTR. Coilin interaction with the telomerase complex protein dyskerin.

Fig. 3. Relative expression levels of ASNS to α-tubulin were dramatically increased when treating human cells with nocodazole and ASNase. Relative expression.

Fig. 7. E2F1 acetylation in A1/A2-KO MEFs

Fig. 7. SCF and IGF-1 accelerate fetal spleen erythropoiesis in vivo.

Fig. 3. Coilin levels correlate with altered nascent U2 snRNA, hTR and rRNA levels.RNA isolated from HeLa or WI-38 cells following RNAi targeting coilin.

P53 is responsible for the induction of apoptosis, developmental malformations and mortality of G1 and G2tert mutant zebrafish. p53 is responsible for.

Combined treatment with LY and sunitinib rescued hypervascularization.

IFN-γ and TNF-α cause an upregulation of Fas expression in human ESCs

Caspase 8 is expressed in human ESCs but is not regulated by IFN-γ, TNF-α or hCG. Caspase 8 is expressed in human ESCs but is not regulated by IFN-γ, TNF-α.

Effects of doxorubicin and epirubicin on proliferation and apoptosis in the hyperplastic livers of transgenic zebrafish larvae. Effects of doxorubicin.

Histone deacetylase inhibitors down-regulate G-protein-coupled estrogen receptor and the GPER-antagonist G-15 inhibits proliferation in endometriotic.

Fig. 6. Effect of SAHA and ML on histone acetylation, BAX, and p21CDKN1A expression.PANC-1 and BxPC-3 cells were incubated for 48 hours with 5 µM.

Fig. 3. Rnd proteins induce stronger responses in subconfluent endothelial cells.HUVECs were transfected with Rnd1, Rnd2, Rnd3 or GFP-encoding plasmids.

Fig. 1. TSA at 165 nM induces maximal acetylation of histone H3 and near-maximal acetylation of histone H4.Immunoblot analysis of acetylated histones H3.

Fig. 4. Co-immunostaining of nocodazole or ASNase treated RPE-1 cells with anti-hASNS and anti-alpha tubulin showed defect in both mitotic spindle formation.

Fig. 1. Mitotic arrest results in differential RNA association with coilin.Untreated or nocodazole treated HeLa cell lysate was used for RNA immunoprecipitations.

Elevated expression of vegfaa in hemangiosarcoma.

Table 1. Relative levels of tRNAMet and tRNASer in human pancreatic adenocarcinoma cell lines.Quantification of tRNAMet and tRNASer in pancreatic adenocarcinoma.

Fig. 3. Reduced histone H3K9 trimethylation in SCNT embryos treated with TSA and VC. (A) Immunostaining analysis indicated that SCNT embryos with TSA+VC.

Fig. 5. Combination of SAHA and ML produces augmented suppression of cellular proliferation with impaired cell cycle progression, enhanced apoptotic.

Fig. 2. TSA impairs growth and disrupts morphogenesis of exocrine pancreas in zebrafish larvae with hyperacetylation of nucleosomal histones.WT zebrafish.

Statistical chart of significantly differentially expressed genes

Fig. 4. Quantitative mRNA expression of two membrane-bound trehalase genes in Harmonia axyridis in response to starvation (0–72 h). Quantitative mRNA expression.

TWEAK increases histone crotonylation in kidney tubular cells.

Ang-1, ESE-1 and Tie-2 expression levels during teratogen-induced CDH

Fig. 11. Chrna6 and Chrnb3 expression depends Pitx3 and Nurr1 but through a different mechanism reported for Vmat2.(A) QPCR analysis of Chrna6 and Chrnb3.

Effect of the plastid translation inhibitor Spec on LR development

Mechanism of Akt1 in promoting reprogramming.

Fig. 4. Perinuclear dynein regulators are not required for primary ciliogenesis.RPE cells were transfected with siRNA and either nocodazole-treated, fixed,

Fig. 1. PARN is differentially expressed in breast cancer versus non-cancerous tissue/cells. PARN is differentially expressed in breast cancer versus non-cancerous.

Effect of Dipy on the expression of chondrogenic markers.

Fig. 4. Impaired induction of TH-response genes in trβ crispants

Effect of the siRNA-mediated knockdown of endogenous MARCH8 on the expression levels of MARCH8 substrates, TfR and CD98, in HepG2 cells. Effect of the.

Morphological changes induced by T3 treatment in trβ crispants

Fig. 2. Expression of Cx43 mutant T154A resulted in non-radial spreading and formation of protrusions in J558µm3 cells spreading in response to BCR signaling.(A)

Fig. 6. RhoB is required for Rnd3-induced stress fibre formation

Fig. 1. Microarray analyses of genes whose expression is regulated by innervation during synaptogenesis.(A) Schematic drawings of the experimental design.

Fig. 1. lgl interacts genetically with Argonaute 1 (AGO1) in the eye.

IL-27 induces expression of multiple IR by CD8+ T cells.

Effects of paclitaxel and vinblastine.

BCL-3 regulates expression of stemness-associated Wnt targets.

Inhibition of macrophage STAT3 activation during ADPKD programming blunts development of the pathological pro-proliferative macrophage phenotype. Inhibition.

Effect of ERα Ub-binding ability on E2-induced receptor transcriptional activity. Effect of ERα Ub-binding ability on E2-induced receptor transcriptional.

Ctip2 directly regulates Notch1 expression in differentiated cells.

The contributions of anti-Ad antibody and T-cell responses to viral clearance in tumor and antitumor efficacy of Ad5 therapy. The contributions of anti-Ad.

A, chemical structures of PRIMA-1 and PRIMA-1Met.

The effects of IAA and glycyrrhizin (Gc) on proliferation and cell-cycle distribution. The effects of IAA and glycyrrhizin (Gc) on proliferation and cell-cycle.

Effect of G9a on the expression of GSH synthesis enzymes.

PNU enhances Ca2+ flux mediated by an engineered variant of the human α7 nAChR. PNU enhances Ca2+ flux mediated by an engineered variant.

Pdcd4 expression inhibits AP-1 transactivation.

SAHA blocks IR-induced increase of RAD51 protein in MM cells.

A, left: LNCaP cells were cultured in media containing complete serum, treated for 24 hours with either ethanol control (0.01%), ABT888 (2.5 mmol/L), or.

Antibody-mediated blockade of the immune-inhibitory PD-1–PD-L1 signaling pathway prolongs survival in poly(I:C)-treated mice. Antibody-mediated blockade.

Hcy decreases VEGFR1/2 and Angs (Ang1, Ang2) gene transcription

Induction of Autophagy Is Dependent on the Delivery of the T3E HopM1.

Presentation transcript:

Fig. 7. ML-60218-mediated suppression of SAHA-induced up-regulation of tRNA as a potential mechanism that contributes to the enhanced anti-cancer actions in pancreatic adenocarcinoma.(A) The tRNAMet and tRNASer levels in PANC-1 and BxPC-3 cells treated for 48 hours as indicated are represented as % of control (untreated or DMSO). ML-60218-mediated suppression of SAHA-induced up-regulation of tRNA as a potential mechanism that contributes to the enhanced anti-cancer actions in pancreatic adenocarcinoma.(A) The tRNAMet and tRNASer levels in PANC-1 and BxPC-3 cells treated for 48 hours as indicated are represented as % of control (untreated or DMSO). Each value is the mean + s.e.m. of three independent experiments, triplicate samples for each PCR, each PCR performed twice, and expressed as % relative to controls. Statistical analysis was performed comparing each experimental group with control, except where indicated. *P<0.05, statistically significant. #P<0.1, trend of statistical significance. NS, not statistically significant. (B) A working model that summarizes the enhanced cytotoxic actions of the combination of ML-60218 and either TSA or SAHA. i, inhibitor; AcH3, acetylated histone H3; AcH4, acetylated histone H4. Nelson S. Yee et al. Biology Open 2012;1:295-307 © 2012. Published by The Company of Biologists Ltd