Modulation of life span by daf-12(null) mutation in animals with reduced DAF-2/InsR activity. Modulation of life span by daf-12(null) mutation in animals.

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Modulation of life span by daf-12(null) mutation in animals with reduced DAF-2/InsR activity. Modulation of life span by daf-12(null) mutation in animals with reduced DAF-2/InsR activity. (A) In the context of DAF-2/InsR activity reduction by daf-2 RNAi, the daf-12(null) mutation shortened median life span [daf-12(null) vs. that of the wild-type, P < 0.0001]. (B) In the context of the Class 1 daf-2(e1368) allele, daf-12(null) shortened the median life span [daf-2(e1368);daf-12(null) vs. daf-2(e1368), P < 0.0001]. (C) The daf-12(null) mutation did not shorten the median life span of animals with DAF-2/InsR activity reduction via the Class 2 daf-2(e1370) allele [daf-2(e1370);daf-12(null) vs. daf-2(e1370), P = 0.4275]. (D) Scatter plot of median survival of daf-12(null) animals normalized to that of daf-12 wild-type animals in the three contexts of reduced DAF-2/InsR activity, separated by assay food source. Error bars indicate SEM. (E and F) Food source control experiments described in (B) and (C), respectively. E. coli HT115 expressing vector control RNAi was used as the assay food source as opposed to E. coli OP50. For each experiment, more than 60 animals were scored per genotype, and at least two experimental replicates were performed. See Table S2 for all raw data and statistics. Kathleen J. Dumas et al. G3 2013;3:841-850 ©2013 by Genetics Society of America