Fig. 8. Sma has a role in ampA pathways controlling cell size and substrate adhesion.(A) Cell volume (µm3) during growth was measured using the volume measurement function of Velocity with 3D reconstructions of Z series using G-actin (DNase I) stained cells. Sma has a role in ampA pathways controlling cell size and substrate adhesion.(A) Cell volume (µm3) during growth was measured using the volume measurement function of Velocity with 3D reconstructions of Z series using G-actin (DNase I) stained cells. Error bars represent standard error of the mean. At least 20 cells were measured per strain in 3 independent experiments on different days. (B) Cell–substrate adhesion indicated by the percent of the cells remaining attached to the substrate after 45 minutes at a shaker speed of 50 rotations per minute (RPM). Error bars represent standard error of the mean. *Indicates a significant difference from the parental strain, with P<0.05 using a Student's t-test. n = 3 rounds of measurements on different days. Jessica S. Kelsey, and Daphne D. Blumberg Biology Open 2013;2:396-406 © 2013. Published by The Company of Biologists Ltd