hStau1 dimerizes on the RNA. hStau1 dimerizes on the RNA. (A, C) Size exclusion chromatography (SEC) profiles of the indicated hStau1 constructs alone (top panels), or pre-incubated with ARF1 SBS (bottom panels). Calculated MW, obtained from MALLS, is indicated in blue. (B, D) EMSA of 5′-fluorescein-labeled ARF1 SBS titrated with the indicated hStau1 constructs. Protein concentrations (in μM) are indicated above the gel (related to Fig 2). Daniela Lazzaretti et al. LSA 2018;1:e201800187 © 2018 Crown copyright. The government of Australia, Canada, or the UK ("the Crown") owns the copyright interests of authors who are government employees. The Crown Copyright is not transferable.