Effects of Electron-Beam Irradiation on Buccal-Cell DNA

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Presentation transcript:

Effects of Electron-Beam Irradiation on Buccal-Cell DNA Philip E. Castle, Montserrat Garcia-Closas, Tracie Franklin, Stephen Chanock, Vinita Puri, Robert Welch, Nathaniel Rothman, Jim Vaught The American Journal of Human Genetics Volume 73, Issue 3, Pages 646-651 (September 2003) DOI: 10.1086/378077 Copyright © 2003 The American Society of Human Genetics Terms and Conditions

Figure 1 Color of Scope before (left) and after (right) sterilizing E-beam irradiation The American Journal of Human Genetics 2003 73, 646-651DOI: (10.1086/378077) Copyright © 2003 The American Society of Human Genetics Terms and Conditions

Figure 2 Results of 989-bp β-globin fragment PCR amplification of DNA extracted from aliquots of buccal cells. The arrow indicates the 989-bp fragment. Lanes 3–12 and 14 (3,4,8, and 10–12 are E-beam irradiated, and 5–7, 9, and 14 are untreated) are test specimens, lanes 13 and 15 are blank controls, lane 2 is a positive amplification control (K562 cell DNA), and lanes 1 and 16 are molecular size standards. Lanes 5, 6, 9, and 14 were rated as “positive,” lanes 3, 4, 7, 8, 10, and 11 were rated as “weak positive,” and lanes 12, 13, and 15 were rated as “negative.” The American Journal of Human Genetics 2003 73, 646-651DOI: (10.1086/378077) Copyright © 2003 The American Society of Human Genetics Terms and Conditions

Figure 3 The effect of E-beam irradiation on the failure rate of detection of STRs as a function of size of the STR for irradiated (□) versus untreated (▴) aliquots. Fragment size (P<.0005), irradiation status (P=.08), and the interaction of fragment size and irradiation status (P=.01) were associated with increased measurement failure. The American Journal of Human Genetics 2003 73, 646-651DOI: (10.1086/378077) Copyright © 2003 The American Society of Human Genetics Terms and Conditions