Pulmonary blood volume measured by contrast enhanced ultrasound: a comparison with transpulmonary thermodilution I.H.F. Herold, M.A. Soliman Hamad, H.C.

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Pulmonary blood volume measured by contrast enhanced ultrasound: a comparison with transpulmonary thermodilution I.H.F. Herold, M.A. Soliman Hamad, H.C. van Assen, R.A. Bouwman, H.H.M. Korsten, M. Mischi British Journal of Anaesthesia Volume 115, Issue 1, Pages 53-60 (July 2015) DOI: 10.1093/bja/aeu554 Copyright © 2015 The Author(s) Terms and Conditions

Fig 1 Schematic overview of the in vitro setup. British Journal of Anaesthesia 2015 115, 53-60DOI: (10.1093/bja/aeu554) Copyright © 2015 The Author(s) Terms and Conditions

Fig 2 (a) Cross-sectional B-mode images of the inflow and outflow tubes obtained by an iE33 ultrasound scanner in the water-filled basin at pump flow of 2 litre min−1 and a true volume of 530 ml. Two regions of interest are drawn in the inflow and outflow tube. At the bottom of the figure, the acoustic dilution curves are presented. They represent the acoustic intensity over time in the selected region of interest. (b) Four chamber view with harmonic imaging; ice-cold SonoVue® is injected via a central venous line and passage through the four heart chambers is acquired with an iE33 TEE-probe. Two regions of interest are drawn in the right atrium and left atrium resulting in acoustic intensity over time signals, which are presented at the bottom of the figure. (c and d) The acoustic intensity dilution curves (A-IDC) of the above-presented B-mode images, both fitted by the LDRW model. The red and yellow lines are the measured A-IDCs, corresponding to panel (a and b). The green and pink curves are the fitted IDCs of the inflow tube and right atrium, and outflow tube and left atrium, the green and pink dotted vertical lines express the MTT of each IDC. British Journal of Anaesthesia 2015 115, 53-60DOI: (10.1093/bja/aeu554) Copyright © 2015 The Author(s) Terms and Conditions

Fig 3 (a) Correlation between ITTV (blue diamonds) and PBV (pink triangles) by PiCCO® as well as ITBVUCA (green circles) with the true volumes in the in vitro setup. Solid lines are lines of regression. (b) Bland-Altman plot of the ITBVUCA (green circles) and the true volume in the in vitro setup and of both ITTV (blue diamonds) and PBV (pink triangles) by PiCCO® with respect to the true volume; solid lines present mean differences (bias); dotted lines represent limits of agreement [bias (1.96 sd)]. (c) Correlation between PBV by PiCCO® and ITBVUCA, in vitro. Solid line is a line of regression. (d) Bland-Altman plot of PBV by PiCCO® and ITBVUCA; solid line is the mean difference (bias); dotted lines are limits of agreement [bias (1.96 sd)]. British Journal of Anaesthesia 2015 115, 53-60DOI: (10.1093/bja/aeu554) Copyright © 2015 The Author(s) Terms and Conditions

Fig 4 (a) Correlation between the PBV by PiCCO® and ITBVUCA by UCA-dilution in patients. Solid line is the line of regression. (b) Bland-Altman plot for repeated measures of the PBV measured by TPTD and ITBVUCA in patients; Solid line is the mean difference (bias); dotted lines are limits of agreement [bias (1.96 sd)]. British Journal of Anaesthesia 2015 115, 53-60DOI: (10.1093/bja/aeu554) Copyright © 2015 The Author(s) Terms and Conditions