Fig. 6. Effect of SAHA and ML-60218 on histone acetylation, BAX, and p21CDKN1A expression.PANC-1 and BxPC-3 cells were incubated for 48 hours with 5 µM.

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Fig. 6. Effect of SAHA and ML-60218 on histone acetylation, BAX, and p21CDKN1A expression.PANC-1 and BxPC-3 cells were incubated for 48 hours with 5 µM SAHA, 100 µM ML-60218, 5 µM SAHA + 100 µM ML-60218, 0.5% DMSO, or untreated (control) and analyzed by immunoblotting. Effect of SAHA and ML-60218 on histone acetylation, BAX, and p21CDKN1A expression.PANC-1 and BxPC-3 cells were incubated for 48 hours with 5 µM SAHA, 100 µM ML-60218, 5 µM SAHA + 100 µM ML-60218, 0.5% DMSO, or untreated (control) and analyzed by immunoblotting. (A) Expression of acetylated histones H3 and H4 was assessed using anti-acetylated histones H3 and H4 antibodies. Anti-total histones H3 and H4 antibodies were used as control. (B) Expression of BAX and p21CDKN1A protein was assessed using anti-BAX and anti-p21CDKN1A antibodies. Anti-actin antibodies were used to indicate equal amount of protein loaded. The data shown are representative of three independent experiments with similar results. Nelson S. Yee et al. Biology Open 2012;1:295-307 © 2012. Published by The Company of Biologists Ltd