Effects of ICER deficiency on MB-cAMP-induced activation of PKA-CREB-VEGF signaling and angiogenesis. Effects of ICER deficiency on MB-cAMP-induced activation.

Slides:

Advertisements

Similar presentations

20,000 GENES IN HUMAN GENOME; WHAT WOULD HAPPEN IF ALL THESE GENES WERE EXPRESSED IN EVERY CELL IN YOUR BODY? WHAT WOULD HAPPEN IF THEY WERE EXPRESSED.

Advertisements

Supplementary Figure S4

Figure 1. WA down-regulates H

Platelet-Derived Growth Factor-BB Mediates Cell Migration through Induction of Activating Transcription Factor 4 and Tenascin-C Kristine P. Malabanan,

Epigallocatechin-3-Gallate Inhibits Matrix Metalloproteinase-9 and Monocyte Chemotactic Protein-1 Expression Through the 67-κDa Laminin Receptor and the.

E2F7/8 and HIF1 are required for hypoxic VEGFA expression.

The effects of doxycycline and micronized purified flavonoid fraction on human vein wall remodeling are not hypoxia-inducible factor pathway-dependent

SESN2 expression in lungs from healthy donors, habitual smokers without COPD and individuals with late-stage COPD. (A) Representative western blots of.

miR-133a positively regulated p53/p21 pathway.

PGE2 promotes angiogenesis through EP4 and PKA Cγ pathway

Interferon (IFN)-Induced Protein 35 (IFI35), a Type I Interferon-Dependent Transcript, Upregulates Inflammatory Signaling Pathways by Activating Toll-Like.

Myocardin-Related Transcription Factors A and B Are Key Regulators of TGF-β1- Induced Fibroblast to Myofibroblast Differentiation Beverly J. Crider, George.

Role of hypoxia-inducible factor-1 alpha in the regulation of plasminogen activator activity in rat knee joint chondrocytes G. Zhu, Y. Tang, X. Liang,

NF-κBp65-specific siRNA inhibits expression of genes of COX-2, NOS-2 and MMP-9 in rat IL-1β-induced and TNF-α-induced chondrocytes Dr C. Lianxu, Ph.D.,

Interferon-γ Protects from Staphylococcal Alpha Toxin-Induced Keratinocyte Death through Apolipoprotein L1 Anne M. Brauweiler, Elena Goleva, Donald Y.M.

Partial knockdown of MPST markedly attenuates while overexpression of MPST exacerbates FFA-induced fat accumulation in hepatocyte in vitro. Partial knockdown.

Vascular endothelial growth factor stimulates protein kinase CβII expression in chronic lymphocytic leukemia cells by Simon T. Abrams, Benjamin R. B. Brown,

BET inhibition and depletion repress the expression of BRCA1 and RAD51

Specificity protein 1 is pivotal in the skin’s antiviral response

Pioglitazone inhibits aromatase expression in human preadipocytes.

Volume 2, Issue 2, Pages (August 2005)

Volume 75, Issue 12, Pages (June 2009)

Role of Sp1 in Transcription of Human ATP2A2 Gene in Keratinocytes

FOXO3a Is Activated in Response to Hypoxic Stress and Inhibits HIF1-Induced Apoptosis via Regulation of CITED2 Walbert J. Bakker, Isaac S. Harris, Tak.

Volume 7, Issue 1, Pages (January 2005)

BTN1 mRNA level in response to extracellular pH determined by comparative RT2PCR. BTN1mRNA level in response to extracellular pH determined by comparative.

Post-ischemic vascular repair mechanisms and the growth factors involved. Post-ischemic vascular repair mechanisms and the growth factors involved. The.

A Drosophila cell culture model to study VAP(P58S) aggregation.

TP53 western blot of primary cultured tail cells from both wild-type (WT) and Tp53Δ11/Δ11 (−/−) rats. TP53 western blot of primary cultured tail cells.

Fig. 1. Lack of Hmga1 and Hmga2 expression in A1/A2-KO mice

Expression of tomoregulin-1 in wild-type and cardiac hypertrophy mouse myocardium. Expression of tomoregulin-1 in wild-type and cardiac hypertrophy mouse.

Working hypothesis of histone lysine crotonylation in kidney injury.

TWEAK downregulates SIRT3 in cultured tubular cells and this is prevented by crotonate. TWEAK downregulates SIRT3 in cultured tubular cells and this is.

IFN-γ and TNF-α cause an upregulation of Fas expression in human ESCs

Silencing of Keap1 reduced the expression of inflammatory markers in ConA-injected livers. Silencing of Keap1 reduced the expression of inflammatory markers.

Reversal of MOR lysosomal targeting by silencing enhanced Rab7 expression. Reversal of MOR lysosomal targeting by silencing enhanced Rab7 expression. A:

Diabetes-induced impairment in angiogenic capacity in subcutaneous sponge implants. Diabetes-induced impairment in angiogenic capacity in subcutaneous.

Fig. 3. Rnd proteins induce stronger responses in subconfluent endothelial cells.HUVECs were transfected with Rnd1, Rnd2, Rnd3 or GFP-encoding plasmids.

Tamoxifen injections induce DNA damage response signaling in αMHC-MerCreMer mice.αMHC-MerCreMer+/+ mice received an injection of 60 μg/g body weight of.

GFP–Sec61b mRNA competes with t-ftz mRNA for the ribosome-binding sites on the ER. (A,B) COS7 cells were transfected with plasmid containing a test gene.

Altered HIF-1α dynamics contribute to reduced VEGF expression in diabetic SIECs. (Aa–c) Total cell contents of HIF-1α, pAkt (at residue S473) and pS6 (at.

Functional classification and visualization of differentially expressed genes. Functional classification and visualization of differentially expressed.

TWEAK increases histone crotonylation in kidney tubular cells.

Detection of gene recombination in endoxifen- or Adeno-Cre-induced tumours. Detection of gene recombination in endoxifen- or Adeno-Cre-induced tumours.

Chronic phenytoin increases activity levels in Mecp2Null/Y mice.

2DG downregulates the expression of HIF-1α, PDK1 and c-Myc in NB xenograft. 2DG downregulates the expression of HIF-1α, PDK1 and c-Myc in NB xenograft.

Ang-1, ESE-1 and Tie-2 expression levels during teratogen-induced CDH

Mechanism of Akt1 in promoting reprogramming.

CREB1 promotes the induction of endogenous ERα target genes.

Effect of the siRNA-mediated knockdown of endogenous MARCH8 on the expression levels of MARCH8 substrates, TfR and CD98, in HepG2 cells. Effect of the.

KIF13A depletion leads to a drop in virus titers, without affecting viral protein expression. KIF13A depletion leadsto a drop in virus titers, without.

Caveolin-1 downregulated survivin expression in cells with enhanced or constitutive activity via the Wnt pathway. Caveolin-1 downregulated survivin expression.

Schematic diagram of the HIF-signalling system and zebrafish homologues. Schematic diagram of the HIF-signalling system and zebrafish homologues. (A) Proteins.

Fig. 6. RhoB is required for Rnd3-induced stress fibre formation

Kinetics of BDNF-induced Erk, Akt and PLCγ activation in the presence of 15 mM NaCl or 15 mM KCl. Representative western blots (A) and quantitative plots.

BCL-3 regulates expression of stemness-associated Wnt targets.

BCL-3 does not mediate β-catenin activity through promoting nuclear translocation or altering levels of LEF1. BCL-3 does not mediate β-catenin activity.

Effect of siRNA transfection on colony formation by Renca cells.

HIF-1 is required for IGF-1-induced VEGF mRNA expression and protein secretion. HIF-1 is required for IGF-1-induced VEGF mRNA expression and protein secretion.

Regulation of Bcl-2 family proteins in the hypothalamus of non-diabetic and diabetic IRS2−/− mice. Regulation of Bcl-2 family proteins in the hypothalamus.

Effect of ERα Ub-binding ability on E2-induced receptor transcriptional activity. Effect of ERα Ub-binding ability on E2-induced receptor transcriptional.

JAK inhibitors suppress IL-17–induced expression of IκB-ζ.

TFAP2A interacts with HIF-1α and regulates VEGF expression and cell proliferation. TFAP2A interacts with HIF-1α and regulates VEGF expression and cell.

CXCR4 expression levels of MDA-MB-231 cells at 48 hours posttransfection of CXCR4 siRNAs. CXCR4 expression levels of MDA-MB-231 cells at 48 hours posttransfection.

TFAP2A knockdown inhibits cell growth by targeting HIF-1α signaling.

Fig. 4 ID1 is a direct CREB target.

Hcy decreases VEGFR1/2 and Angs (Ang1, Ang2) gene transcription

RGS16 expression is decreased in chondrosarcoma and RGS16 transfection counteracts the effects of miR-181a on VEGF and MMP1 expression. RGS16 expression.

CREB1 binds at the proximal region of the TGFB2 promoter and induces its transcriptional activation. CREB1 binds at the proximal region of the TGFB2 promoter.

Glial cell line-derived neurotrophic factor (GDNF) induces hepatic stellate cell (HSC) activation via the activin receptor-like kinase 5 (ALK5)/Smad pathway.

Presentation transcript:

Effects of ICER deficiency on MB-cAMP-induced activation of PKA-CREB-VEGF signaling and angiogenesis. Effects of ICER deficiency on MB-cAMP-induced activation of PKA-CREB-VEGF signaling and angiogenesis. Diabetic SIECs were rendered ICER1 deficient (ICER1 siRNA) by using siRNA. Transfection efficiency was confirmed using TaqMan real-time PCR and western blotting. After culturing for 48 hours, cells were exposed to MB-cAMP (100 μM) for various time intervals and were then used to evaluate pCREB-DNA binding activity (Aa, MB-cAMP for 2 hours), CRE transcriptional activity (Ab, MB-cAMP for 6 hours), mRNA expression of CRE target genes (Ba-c, MB-cAMP for 2 hours), HIF-1α-HRE binding affinity (C, MB-cAMP for 2 hours), VEGF promoter activity (Da, MB-cAMP for 24 hours), VEGF mRNA (Db, MC-cAMP for 6 hours) and VEGF protein expression (Dc, MB-cAMP for 24 hours). ‘C-ECs’, control sponge implant endothelial cells; ‘D-ECs’, diabetic sponge endothelial cells. Results are expressed as means±s.e.m. for three independent experiments. *Significantly different from corresponding control values at P≤0.05. **Significantly different from corresponding MB-cAMP-treated control values at P≤0.05. ∫Significantly different from corresponding D-ECs MB-cAMP-treated siRNA control values at P≤0.05. Milad S. Bitar, and Fahd Al-Mulla Dis. Model. Mech. 2015;8:65-80 © 2015. Published by The Company of Biologists Ltd