Fig. 1. Fusion protein amino acid sequences. Chick CaV2 Fig. 1. Fusion protein amino acid sequences.Chick CaV2.2 I–II loop (I–II; (A)) and II–III loop distal half (II–IIIdistal; (B)) fusion protein amino acid sequences aligned with the corresponding region of the native long splice-variant chick CaV2.2 (cdB1; accession number AAD51815) used for cloning. Fusion protein amino acid sequences.Chick CaV2.2 I–II loop (I–II; (A)) and II–III loop distal half (II–IIIdistal; (B)) fusion protein amino acid sequences aligned with the corresponding region of the native long splice-variant chick CaV2.2 (cdB1; accession number AAD51815) used for cloning. Both constructs have N terminal GST (not shown) and C terminal FLAG (grey highlight) tags. (C) Chick CaV2.2 distal C-terminus fusion protein (C3strep) amino acid sequence aligned with long splice CaV2.2 variant (cdB1, as above). Sabiha R. Gardezi et al. Biology Open 2013;2:492-498 © 2013. Published by The Company of Biologists Ltd