Comparison of rhinovirus antibody titers in children with asthma exacerbations and species-specific rhinovirus infection Jua Iwasaki, BSc (Hons), Wendy-Anne.

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Presentation transcript:

Comparison of rhinovirus antibody titers in children with asthma exacerbations and species-specific rhinovirus infection Jua Iwasaki, BSc (Hons), Wendy-Anne Smith, PhD, Siew-Kim Khoo, BSc (Hons), Joelene Bizzintino, PhD, Guicheng Zhang, PhD, Des W. Cox, MD, Ingrid A. Laing, PhD, Peter N. Le Souëf, MD, Wayne R. Thomas, PhD, Belinda J. Hales, PhD Journal of Allergy and Clinical Immunology Volume 134, Issue 1, Pages 25-32.e1 (July 2014) DOI: 10.1016/j.jaci.2014.03.014 Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 IgG1 antibody binding to VP1 antigens representing HRV-A (HRV-A34 and HRV-A1B), HRV-B (HRV-B14 and HRV-B69), and HRV-C (HRV-C3 and HRV-C5) as well as HPV Sabin in 96 ED asthmatic children (filled circles) and 47 nonasthmatic control children (open circles). A, Total IgG1 binding (ng/mL). B, Species-specific IgG1 binding (ng/mL). The median and interquartile range are indicated. Species-specific titers to HRV-A were significantly higher than other antigens in both ED asthmatic children and nonasthmatic control children (P < .001) as determined using the related-samples Wilcoxon signed-rank test. Comparisons between ED asthmatic children and nonasthmatic controls were analyzed using the Mann-Whitney test. The prevalence (%) of subjects with titers below the assay limit of 500 ng/mL is indicated for each antigen. Prevalence between the 2 groups was analyzed using the χ2 test. *P < .05. Journal of Allergy and Clinical Immunology 2014 134, 25-32.e1DOI: (10.1016/j.jaci.2014.03.014) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 IgG1 antibody binding to HRV-A and HRV-C in children with a known HRV infection. ED asthmatic children infected with HRV-A at presentation to hospital (n = 19, pointed circles) did not have significantly different titers to ED asthmatic children with no detectable HRV-A (n = 54, closed circles). ED asthmatic children with no detectable HRV-A had significantly higher anti–HRV-A titers than did nonasthmatic control children with no detectable HRV-A (n = 35, open circles) with the P value indicated. ED asthmatic children infected with HRV-C (n = 47, pointed squares) have low species-specific IgG1 titers to HRV-C, which were not significantly different to ED asthmatic children with no detectable HRV-C at the time of their acute asthma exacerbation (n = 45, filled squares). The anti–HRV-C response in ED asthmatic children was not significantly different from that in nonasthmatic control children with no detectable HRV-C at the time of recruitment (n = 34, open squares). The median and interquartile range are indicated. Journal of Allergy and Clinical Immunology 2014 134, 25-32.e1DOI: (10.1016/j.jaci.2014.03.014) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Species-specific IgG1 binding to HRV-C (HRV-C3) and HRV-A (HRV-A34) antigens for plasma from 30 ED asthmatic children with a known HRV-C infection at the time of hospitalization. The IgG1 titers for each individual immediately following exacerbation from asthma (acute) and at convalescence (median, 12 weeks) are indicated. Journal of Allergy and Clinical Immunology 2014 134, 25-32.e1DOI: (10.1016/j.jaci.2014.03.014) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Specific IgG1 binding to HRV-A (HRV-A34), HRV-B (HRV-B14), and HRV-C (HRV-C3) in relation to age in ED asthmatic children following acute asthma exacerbation (n = 96) (A) and nonasthmatic control children (n = 47) (B). As determined by the Spearman rho test, there was a significant positive correlation between age and IgG1 titers for HRV-A and HRV-B in ED asthmatic children. Journal of Allergy and Clinical Immunology 2014 134, 25-32.e1DOI: (10.1016/j.jaci.2014.03.014) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 Isolate-specific IgG1 titers in 2 HRV-infected subjects following asthma exacerbation and at convalescence. Results are represented as the percentage of isolate-specific response to species-specific response. Both HRV isolates were HRV-A. The species-specific titer of subject 1 to the isolate was 2,687,584 ng/mL at asthma exacerbation and 1,976,063 ng/mL at convalescence. The species-specific titer of subject 2 to the isolate was 110,739 ng/mL at asthma exacerbation and 1,086,187 ng/mL at convalescence. Journal of Allergy and Clinical Immunology 2014 134, 25-32.e1DOI: (10.1016/j.jaci.2014.03.014) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions