F. B. Spencker, S. Haupt, M. C. Claros, S. Walter, T. Lietz, R

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Epidemiologic characterization of Pseudomonas aeruginosa in patients with cystic fibrosis F.B. Spencker, S. Haupt, M.C. Claros, S. Walter, T. Lietz, R. Schille, A.C. Rodloff Clinical Microbiology and Infection Volume 6, Issue 11, Pages 600-607 (November 2000) DOI: 10.1046/j.1469-0691.2000.00171.x Copyright © 2000 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 1 PFGE patterns of 31 strains from patient 4. The patient harbors three clones: clone 7 (lanes 2, 6, 9, and 16), clone 8 (lanes 2, 3, 28, and 31), and clone 6 with two clonal variants (the remaining lanes). Strains 23, 28 and 31 differ in more than six bands from strains 2, 6, 9 and 16, and therefore were assigned a separate PFGE number. Clones 7 and 8 show the same pyocintype 5/x(S5), and clone 6 has pyocintype 1. L is the molecular size marker (PFG Marker, New England Biolabs, 50–100 kb). See also Figure 2. Clinical Microbiology and Infection 2000 6, 600-607DOI: (10.1046/j.1469-0691.2000.00171.x) Copyright © 2000 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 2 Dendrogram of similarities of 31 fragment patterns from patient 4. Strain numbers correspond with lanes in Figure 3. The patient is co-colonized by strains of two major clusters. The upper cluster is formed by clones 6A and 6B. The lower cluster represents clones 7 and 8. The similarity analysis suggests clonal relatedness between clones 7 and 8, despite differences in more than four bands. Clinical Microbiology and Infection 2000 6, 600-607DOI: (10.1046/j.1469-0691.2000.00171.x) Copyright © 2000 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 3 PFGE patterns of five P. aeruginosa isolates from patient 29 (lanes 1–5) and two isolates from his brother, patient 30 (lanes 6 and 7). The typing results for the strains are summarized in Table 1. L is the molecular size marker (PFG marker, New England Biolabs, 50–100 kb). Clinical Microbiology and Infection 2000 6, 600-607DOI: (10.1046/j.1469-0691.2000.00171.x) Copyright © 2000 European Society of Clinical Infectious Diseases Terms and Conditions