Fig. 8. Lhx1-RNAi perturbs NR formation

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Fig. 8. Lhx1-RNAi perturbs NR formation Fig. 8. Lhx1-RNAi perturbs NR formation.(A–E) Hematoxylin-Eosin staining at stage 24. Lhx1-RNAi perturbs NR formation.(A–E) Hematoxylin-Eosin staining at stage 24. (B) Magnified view of the posterior retina in (A); distinct pigmentation is observed on the basal side of the single-layered RPE. (C) Small eye phenotype after Lhx1-RNAi, in which a pigmented vesicle (pv) forms and lens (le) development is perturbed. (D) The optic epithelium abutting the lens. (E) Posterior optic epithelium with distinct polarized pigmentation. (F–M) Expression of Chx10, Otx2, Mitf, and Mmp115. Eyes were examined at 24 hours (F,G,J,K), 36 hours (H,L) or 60 hours post-electroporation (I,M). In situ hybridization was performed under the same conditions for controls and Lhx1-RNAi, depending on the probes. (J) Chx10 expression in the inner layer of the optic cup is reduced (arrowhead). (K) The Otx2 expression domain is expanded to the inner layer of the optic cup (arrowhead). (L) Mitf expression is detected in the inner layer as well as outer layer of the optic cup. (M) Mmp115 expression is detected in the dorsal epithelium of the OV (arrowheads) and abutting the lens vesicle (arrow). The axis of the eye is depicted as follows: A, anterior; D, dorsal; P, posterior; V, ventral; nr, neural retina; and rpe, retinal pigmented epithelium. Scale bars, 100 µm (A,C), 10 µm (B,D,E). Takumi Kawaue et al. Biology Open 2012;1:1083-1093 © 2012. Published by The Company of Biologists Ltd