Fig. 3. (A) Effect of bilobetin on the protein level for lipogenic regulators. Simian virus 40 T (SV40T)-transformed sebocytes were treated with insulin-like.

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Presentation transcript:

Fig. 3. (A) Effect of bilobetin on the protein level for lipogenic regulators. Simian virus 40 T (SV40T)-transformed sebocytes were treated with insulin-like growth factor-1 (IGF-1) and bilobetin at the indicated concentrations for 1 day. Protein level for lipogenic regulators was evaluated by Western blot. (B) Effect of bilobetin on intracellular signaling. Cells were pretreated with bilobetin at the indicated concentrations for 30 minutes, and then IGF-1 was treated for 30 minutes. Cellular proteins were prepared and phosphorylation of signaling molecules was evaluated by Western blot. Actin was used as a loading control. Western bands were densitometrically analyzed and normalized with actin. SREBP: sterol response element binding protein, FDFT1: farnesyl-diphosphate farnesyltransferase 1, SCD: stearoyl-CoA desaturase. Fig. 3. (A) Effect of bilobetin on the protein level for lipogenic regulators. Simian virus 40 T (SV40T)-transformed sebocytes were treated with insulin-like growth factor-1 (IGF-1) and bilobetin at the indicated concentrations for 1 day. Protein level for lipogenic regulators was evaluated by Western…. Ann Dermatol. 2019 Jun;31(3):294-299. https://doi.org/10.5021/ad.2019.31.3.294