Figure 1. Workflow of the analysis to estimate the number of true human miRNAs. Samples containing NGS data were ... Figure 1. Workflow of the analysis.

Slides:

Advertisements

Similar presentations

Figure 1. Unified models predicting gene regulation based on landscapes of gene-regulating factors. For each gene, position specific combinatorial patterns.

Advertisements

From: Learning by Working in Big Cities

Fig. 1 Nodes in a conceptual knowledge graph

Figure 1: Pneumomediastinum and subcutaneous emphysema as indicated by the arrows. From: Pneumomediastinum and subcutaneous emphysema after successful.

Figure 1. drb7.2 mutant plants display altered accumulation of endoIR-siRNA. Wild-type (Col-0) and drb7.2 mutant plants were subjected to high throughput.

Figure 1. The flow chart illustrates the construction process of anti-CRISPRdb, and the information that users can obtain from anti-CRISPRdb. From: Anti-CRISPRdb:

Figure 1: Necrobiosis lipoidica: Yellow-brown skin lesions with indurated borders located on both lower legs. Figure 1: Necrobiosis lipoidica: Yellow-brown.

Figure 1. (A) The VEGF promoter PQS and scheme of G oxidation to OG, as well as (B) the proposed APE1-dependent pathway ... Figure 1. (A) The VEGF promoter.

Figure 1. Circular taxonomy tree based on the species that were sequenced in our study. Unless provided in the caption above, the following copyright applies.

ADAb: anti-drug antibody.

Figure 1. Overview of the workflow of NetworkAnalyst 3.0.

FIGURE 1 Kidney biopsies of Patients 1 and 2

Figure 1: Axial T2 W images of penis showed a well-defined septated area of 2 cm in the posterior aspect of ... Figure 1: Axial T2 W images.

FIGURE 1 Histological diagnoses divided into 8-year time frames (n = 1208). Unless provided in the caption above, the following copyright applies to the.

FIGURE 1 The effect of daprodustat on hemoglobin (Hgb) levels

Figure 1. BRCA1-associated R-Loop accumulation at a non-coding region upstream of ESR1 locus. (A) Alignment of DRIP-seq ... Figure 1. BRCA1-associated.

FIGURE 2 Responses to the question: regarding vasoactive drugs, does your centre use the following frequently, rarely ... FIGURE 2 Responses to the question:

FIGURE 1 Maternal (A) urinary aldosterone; (B) plasma active renin; (C) urinary AGT concentrations; and (D) plasma AGT ... FIGURE 1 Maternal (A) urinary.

FIGURE 1 Flow chart of patient enrollment and exclusion

Figure 1. Ratios of observed to expected numbers of exon boundaries aligning to boundaries of domain and disorder ... Figure 1. Ratios of observed to expected.

Figure 1. autoMLST workflow depicting placement and de novo mode

Fig. 1 Kaplan-Meier plot of cumulative incidence of cancer onset following dermatomyositis diagnosis stratified ... Anti-TIF1-Ab: anti-transcriptional.

Figure 1. Chemical structures of DNA and tc-DNA

Figure 1 Flortaucipir PET MUBADA/PERSI SUVr at baseline, 9 and 18 months for individual subjects. Each subject is ... Figure 1 Flortaucipir PET MUBADA/PERSI.

Fig. 1 Mean change from baseline in ANC ± s. e

Point estimates with ... Point estimates with 95% CI. HR: hip replacement; KR: knee replacement. Unless provided in the caption above, the following copyright.

FIGURE 1 Participant flow diagram. Exercise Counseling Clinic (ECC).

Graph 1. The number of homicide cases per year discussing neuro-evidence. Unless provided in the caption above, the following copyright applies to the.

Figure 6. The DNA lyase activity of hNTHL1 contributes to the processing of lesions in nucleosomes, even in the ... Figure 6. The DNA lyase activity of.

Figure 1. Analysis of human TRIM5α protein with Blast-Search and PhyML+SMS ‘One click’ workflow. (A) NGPhylogeny.fr ... Figure 1. Analysis of human TRIM5α.

Figure 2. Natural history of chlamydia transmission, with arrows showing the transitions between health states. Figure 2. Natural history of chlamydia.

Figure 1 Nelson-Aalen estimates of the cumulative incidence rates for patients on versus off IST. ON = optic neuritis; ... Figure 1 Nelson-Aalen estimates.

FIGURE 1 Study consort diagram

Fig. 2 Case 2. Levels of serum creatinine and anti-GBM antibodies before and during treatment with cyclophosphamide, ... Fig. 2 Case 2. Levels of serum.

Figure 1. Illustration of DGR systems and their prediction using myDGR

Figure 1. The pipeline of Aggrescan3D 2.0 server.

FIGURE 1 Food groups consumed (mean, g/d) among US infants and young children by age group and tertile of mean ... FIGURE 1 Food groups consumed (mean,

Figure 1. The workflow of Cistrome-GO

Figure 1. Prediction result for birch pollen allergen Bet v 1 (PDB: 1bv1), as obtained by comparison to the cherry ... Figure 1. Prediction result for.

Figure 1. Using Voronoi tessellation to define contacts

Figure 4. Levels of cerebrospinal fluid (CSF) immune and inflammatory markers in participants grouped by CSF human ... Figure 4. Levels of cerebrospinal.

Figure 4. RLS spectra of (A) TMPipEOPP and (B) OMHEPzEOPP in the presence of different concentrations of KRAS. The RLS ... Figure 4. RLS spectra of (A)

Figure 1. PaintOmics 3 workflow diagram

Figure 1. Concept of poly(A) tail labeling for translation and localization analyses of reporter mRNAs. Azido-modified ... Figure 1. Concept of poly(A)

Figure 1. Schematic diagram of solar energy and coal-fired power generation system. Unless provided in the caption above, the following copyright applies.

Figure 1. Uncertainty reduction, value creation, and appropriation in two case studies. Unless provided in the caption above, the following copyright applies.

Figure 1 Ratio of the geometric mean concentration of hsTnT (A) and sST2 (B) at baseline (BL) and each subsequent ... Figure 1 Ratio of the geometric mean.

Figure 1. MERMAID web server interface (Start page, Parameter page): MERMAID provides two ways to submit a protein ... Figure 1. MERMAID web server interface.

Figure 1. Yvis platform overview

Figure 1. The framework of NetGO with seven steps

Figure 1. Workflow of the HawkDock server that is divided into three major steps: (i) input of unbound or bound protein ... Figure 1. Workflow of the HawkDock.

Figure 1 Patient disposition

Figure 1 BM community in the normozoospermic and iNOA human testis parenchyma. Quantification of the 16 S copies/ng in ... Figure 1 BM community in the.

Figure 1. Trial profile. (A) Part A and (B) part B

Figure 1. Scheme of a phosphorothioated-terminal hairpin formation and self-priming extension (PS-THSP) for selection ... Figure 1. Scheme of a phosphorothioated-terminal.

Figure 1. Excess cost of methicillin-resistant Staphylococcus aureus (MRSA) compared with methicillin-susceptible S. ... Figure 1. Excess cost of methicillin-resistant.

Figure 1. 3C analysis of HEM3, BLM10, and SEN1 genes in rpb4Δ and isogenic wild type cells. (A) Schematic ... Figure 1. 3C analysis of HEM3, BLM10, and.

Figure 1 Genetic results. No case had more than one diagnostic result

Figure 1. CSB does not affect the recruitment of OGG1 to oxidative DNA damage. (A) Representative stills of time-lapse ... Figure 1. CSB does not affect.

Figure 1. (A) Baseline contrast-enhanced CT scan of melanoma patient presenting with metastases in the liver and lymph ... Figure 1. (A) Baseline contrast-enhanced.

Figure 1. Prevalence of parasitic infection and anemia among the children. Unless provided in the caption above, the following copyright applies to the.

Figure 1. Illustration of aggregation functions on the local network of 1-decene. 1-decene is marked as target. ... Figure 1. Illustration of aggregation.

Figure 1. Optimization of the variant calling algorithm, ADIScan1, by tangential conversion of read depth ratios ... Figure 1. Optimization of the variant.

Figure 1. GWAS Catalog associations for coronary artery disease plotted across all chromosomes. Associations added ... Figure 1. GWAS Catalog associations.

Figure 1 The workflow of CAR development from a hybridoma

Source:Zimnisky (2014). Source:Zimnisky (2014).

Figure 1 Mechanisms of mitral regurgitation.

Fig. 1. —GO categories enriched in gene families showing high or low omega (dN/dS) values for Pneumocystis jirovecii. ... Fig. 1. —GO categories enriched.

Figure 1. (A) Overview of ENPD including data source, data processing and features. Transcriptomes from TSA, genomes ... Figure 1. (A) Overview of ENPD.

Figure 1. Hepatitis C screening and diagnostic algorithm at the MSF clinic, Karachi, Pakistan, March 2016–September ... Figure 1. Hepatitis C screening.

Presentation transcript:

Figure 1. Workflow of the analysis to estimate the number of true human miRNAs. Samples containing NGS data were ... Figure 1. Workflow of the analysis to estimate the number of true human miRNAs. Samples containing NGS data were collected from SRA, TCGA and data uploaded to miRMaster, and the obtained samples and reads were filtered. Three sets of miRNAs were created: miRBase high-confidence (HC), miRBase low-confidence set (LC) and other (Other). Using in silico high-throughput validation and experimental low-throughput validation steps, the probabilities that a miRNA will pass the validation procedure have been calculated for each miRNA set respectively. Finally, the number of true miRNAs was estimated using the original miRNA counts and the computed probabilities. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com Nucleic Acids Res, Volume 47, Issue 7, 01 March 2019, Pages 3353–3364, https://doi.org/10.1093/nar/gkz097 The content of this slide may be subject to copyright: please see the slide notes for details.

Figure 2. Northern blots of endogenous miRNAs Figure 2. Northern blots of endogenous miRNAs. (A) The 11 analyzed cell lines that are indicated on top of the figure ... Figure 2. Northern blots of endogenous miRNAs. (A) The 11 analyzed cell lines that are indicated on top of the figure were derived from lung (A549), liver (HUH-7), bone marrow (SHSY-5Y), keratinocytes (HaCaT), cervix (HeLa), mammary gland (MCF7), placenta (JEG-3), Testis (Tera-1), prostate (PC-3), B-lymphocytes (DG-75) and T-lymphocytes (Jurkat). HEK-293T RNA was used as a reference to compare signals to exogenously expressed miRNAs. The endogenous mature forms are shown for the miRNAs indicated on the left side of the figure. (B) The number of mature and premature forms of the endogenous miRNA expressed in the 12 cells lines as indicated in Figure 2A. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com Nucleic Acids Res, Volume 47, Issue 7, 01 March 2019, Pages 3353–3364, https://doi.org/10.1093/nar/gkz097 The content of this slide may be subject to copyright: please see the slide notes for details.

Figure 3. Representative NB results for a positive, questionable and negative miRNA in HEK 293T cells. (A) NB for ... Figure 3. Representative NB results for a positive, questionable and negative miRNA in HEK 293T cells. (A) NB for hsa-miR-155–5p from high-confidence set A showing distinct bands for its precursor (p) and mature (m) form. (B) Hybridization against hsa-miR-1260a (low-confidence set B) detects two small RNA fragments with similar signal intensities for the control. (C) Probing for hsa-miR-6776–5p (low-confidence set B) did not result in any specific bands. Ethidium bromide staining of RNA gels was used as a loading control. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com Nucleic Acids Res, Volume 47, Issue 7, 01 March 2019, Pages 3353–3364, https://doi.org/10.1093/nar/gkz097 The content of this slide may be subject to copyright: please see the slide notes for details.

Figure 4. Comparison of exogenous and endogenous miRNA expression by northern blotting. The mature form is indicated by ... Figure 4. Comparison of exogenous and endogenous miRNA expression by northern blotting. The mature form is indicated by ‘m’ and the premature form by ‘p’. The left part of the figure shows exogenous expression of miR-148a-3p in HEK 293T cells. HEK 293T cells that were transfected with an empty vector are shown as a control (ctrl). The right part of the figure shows endogenous expression of miR-148a-3p in 12 cells lines as specified in Figure 2A. To show endogenous miRNAs, the signal intensity of the endogenous miRNAs apparently is enhanced as compared to the signal intensity of the exogenous miRNAs due to the very strong signal of overexpressed miR-148a-3p (compare backgrounds of exo- and endogenous northern blots and see the comparison between HEK 293T cells that were used as a control for the transfection analysis (ctrl) shown in the left part of the figure and the HEK 293T cells that were compared with other cells shown in the right part of the figure). As described in the ‘Materials and Methods’ section, contrast and brightness were adjusted by the software during scanning according to the darkest spot on the blot. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com Nucleic Acids Res, Volume 47, Issue 7, 01 March 2019, Pages 3353–3364, https://doi.org/10.1093/nar/gkz097 The content of this slide may be subject to copyright: please see the slide notes for details.