Volume 55, Issue 3, Pages 963-975 (March 1999) Sodium reabsorption and distribution of Na+/K+-ATPase during postischemic injury to the renal allograft Osun Kwon, Geraldine Corrigan, Bryan D. Myers, Richard Sibley, John D. Scandling, Donald Dafoe, Edward Alfrey, W. James Nelson Kidney International Volume 55, Issue 3, Pages 963-975 (March 1999) DOI: 10.1046/j.1523-1755.1999.055003963.x Copyright © 1999 International Society of Nephrology Terms and Conditions
Figure 1 Distribution of Na+/K+-ATPase in proximal convoluted tubule cells (PCT), grading of the severity of injury. The injury is heterogeneous, and the PCT in each panel, which exemplifies the degree of injury being described, is designated PCT1. Abbreviations are: *, distal straight tubule; DCT, distal convoluted tubule; PST, proximal straight tubule. Grades are defined as: Grade 1 (A), normal, basolateral staining; Grade 2 (B), mild injury, diminished basolateral staining with some cytoplasmic redistribution; Grade 3 (C), moderate injury, further diminution of basolateral staining with increased cytoplasmic redistribution; Grade 4 (D), severe injury, virtually complete cytoplasmic redistribution. Kidney International 1999 55, 963-975DOI: (10.1046/j.1523-1755.1999.055003963.x) Copyright © 1999 International Society of Nephrology Terms and Conditions
Figure 2 Creatinine clearance (CCr) in the first post-transplant week. Symbols are: (•) sustained ARF; (○) recovering ARF. Values are expressed as mean ± SEM. *P < 0.05 on postoperative day 7 vs. postoperative day 0 within each group; **P < 0.05 sustained vs. recovering ARF. The shaded area indicates the corresponding range for GFR in an optimally functioning transplanted kidney[15]. Kidney International 1999 55, 963-975DOI: (10.1046/j.1523-1755.1999.055003963.x) Copyright © 1999 International Society of Nephrology Terms and Conditions
Figure 3 Fractional excretion of sodium (A) and lithium (B) in subjects with sustained (•) and recovering (○) acute renal failure (ARF) in the first post-transplant week. Values are expressed as mean ± SEM. *P < 0.05 day 7 vs. day 0 within each group; **P < 0.05 sustained vs. recovering ARF. Kidney International 1999 55, 963-975DOI: (10.1046/j.1523-1755.1999.055003963.x) Copyright © 1999 International Society of Nephrology Terms and Conditions
Figure 4 Electron photomicrograph of proximal tubule in subject with sustained acute renal failure (ARF). Notice the reduction of apical brush border and basal infoldings. Kidney International 1999 55, 963-975DOI: (10.1046/j.1523-1755.1999.055003963.x) Copyright © 1999 International Society of Nephrology Terms and Conditions
Figure 5 Nephron segment localization by staining with CHIP28 (left) and cytokeratin K8 (middle) and Tamm Horsfall protein (right) in cortex (upper panels) and medulla (lower panels) in biopsy tissue from a healthy living kidney donor (control). Abbreviations are: PCT, proximal convoluted tubule; PST, proximal straight tubule; CD, collecting duct; *, distal straight tubules (thick ascending limb) are identified by positive staining for Tamm-Horsfall protein. Kidney International 1999 55, 963-975DOI: (10.1046/j.1523-1755.1999.055003963.x) Copyright © 1999 International Society of Nephrology Terms and Conditions
Figure 6 Distribution along the nephron of Na+/K+-ATPase, CHIP28 (Aquaporin-1), cytokeratin K8, and Tamm-Horsfall protein (THP). The presence of a line indicates staining, and its breadth indicates the intensity of staining at each site. Abbreviations are: CD, collecting duct; DCT, distal convoluted tubule; DST, distal straight tubule (thick ascending limb); DTL and ATL, descending and ascending thin limbs of Henle's loop; Glom, glomerulus; IMCD, inner medulla collecting duct; PCT, proximal convoluted tubule; PST, proximal straight tubule. Kidney International 1999 55, 963-975DOI: (10.1046/j.1523-1755.1999.055003963.x) Copyright © 1999 International Society of Nephrology Terms and Conditions
Figure 7 Grading of the distribution of Na+/K+-ATPase in proximal convoluted (A) and proximal straight (B) tubule cells on the day of transplantation. Symbols are (▪) sustained ARF; (▧) recovering ARF. Kidney International 1999 55, 963-975DOI: (10.1046/j.1523-1755.1999.055003963.x) Copyright © 1999 International Society of Nephrology Terms and Conditions
Figure 8 Grading of the distribution of Na+/K+-ATPase in distal straight (A), distal convoluted (B), and collecting duct (C) tubule cells on the day of transplantation. Symbols are: (▪) sustained ARF; (▧) recovering ARF. Kidney International 1999 55, 963-975DOI: (10.1046/j.1523-1755.1999.055003963.x) Copyright © 1999 International Society of Nephrology Terms and Conditions
Kidney International 1999 55, 963-975DOI: (10. 1046/j. 1523-1755. 1999 Kidney International 1999 55, 963-975DOI: (10.1046/j.1523-1755.1999.055003963.x) Copyright © 1999 International Society of Nephrology Terms and Conditions
Kidney International 1999 55, 963-975DOI: (10. 1046/j. 1523-1755. 1999 Kidney International 1999 55, 963-975DOI: (10.1046/j.1523-1755.1999.055003963.x) Copyright © 1999 International Society of Nephrology Terms and Conditions
Kidney International 1999 55, 963-975DOI: (10. 1046/j. 1523-1755. 1999 Kidney International 1999 55, 963-975DOI: (10.1046/j.1523-1755.1999.055003963.x) Copyright © 1999 International Society of Nephrology Terms and Conditions