Selective insulin-like growth factor-I antagonist inhibits mouse embryo development in a dose-dependent manner Jose Inzunza, Ph.D., Olle Danielsson,

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Presentation transcript:

Selective insulin-like growth factor-I antagonist inhibits mouse embryo development in a dose-dependent manner Jose Inzunza, Ph.D., Olle Danielsson, M.D., Ph.D., Parameswaran Grace Lalitkumar, Ph.D., Olle Larsson, M.D., Ph.D., Magnus Axelson, M.D., Ph.D., Virpi Töhönen, Ph.D., Kristina Gemzell Danielsson, M.D., Ph.D., Anneli Stavreus-Evers, Ph.D. Fertility and Sterility Volume 93, Issue 8, Pages 2621-2626 (May 2010) DOI: 10.1016/j.fertnstert.2009.12.044 Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Immunohistochemistry images show staining of phosphorylated and nonphosphorylated IGF-I receptor. (A to F) Staining of phosphorylated receptor. (H to M) Staining of nonphosphorylated of the following: oocyte, one-cell, two-cell, four-cell, eight-cell, and blastocyst. (G and N) Negative control. Staining of IGF-I receptor is shown in green, and nuclear staining using DAPI is shown in blue. Fertility and Sterility 2010 93, 2621-2626DOI: (10.1016/j.fertnstert.2009.12.044) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Relative expression of IGF-IR mRNA in mouse oocytes and mouse preimplantation embryos is shown. Fertility and Sterility 2010 93, 2621-2626DOI: (10.1016/j.fertnstert.2009.12.044) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Embryo development after exposure to picropodophyllin for 24 hours at the one-cell stage. (A) Embryos treated with picropodophyllin for 24 hours at the one-cell stage and thereafter cultured in KSOM media for 72 hours, after a total 96 hours. (B) Embryos treated with picropodophyllin for 24 hours at the two-cell stage and thereafter cultured in KSOM media for 72 hours. (C) Embryos treated with picropodophyllin for 24 hours at the four-cell stage and thereafter cultured in KSOM media for 48 hours. (D) Embryos treated with picropodophyllin for 24 hours at the eight-cell stage and thereafter cultured in KSOM media for 24 hours. (E) Control embryos without treatment at expected blastocyst stage after 96 hours in culture. Fertility and Sterility 2010 93, 2621-2626DOI: (10.1016/j.fertnstert.2009.12.044) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions