Heatmap of relative expression levels of 75 O

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Heatmap of relative expression levels of 75 O Heatmap of relative expression levels of 75 O. virilis miRNAs in hepatopancreas tissue under anoxia. miRNA expression was measured in hepatopancreas tissue of crayfish under 2 h and 20 h anoxic stress relative to that in normoxic conditions using qPCR. Heatmap of relative expression levels of 75 O. virilis miRNAs in hepatopancreas tissue under anoxia. miRNA expression was measured in hepatopancreas tissue of crayfish under 2 h and 20 h anoxic stress relative to that in normoxic conditions using qPCR. Data are means±s.e.m. of four independent biological replicates. Statistical analyses used a one-way ANOVA with Dunnett's post hoc test: *P<0.05, **P<0.01, ***P<0.001. Refer to Table S2 for the relative expression ±s.e.m. values of all 76 miRNA species. Simon G. English et al. J Exp Biol 2018;221:jeb190579 © 2018. Published by The Company of Biologists Ltd