SDS-PAGE (A,E,I) and immunoblot analysis (B–D, F–H, J–L) of SEC elution profiles of crystallins from D. mawsoni (A–D), T. obesus (E–H) and B. taurus (I–L). SDS-PAGE (A,E,I) and immunoblot analysis (B–D, F–H, J–L) of SEC elution profiles of crystallins from D. mawsoni (A–D), T. obesus (E–H) and B. taurus (I–L). The primary antibodies used for detection are denoted on the left. Molecular mass markers (Lane M; kDa) are denoted on the left of each panel. Lane numbers correspond to the locations of the numbered positions on Fig. 2. for each species. All immunoblots are independent replicas of SDS-PAGE presented in Fig. 3A,E,I. Andor J. Kiss et al. J Exp Biol 2004;207:4633-4649 © The Company of Biologists Limited 2004