Fig. 10. A mutant clone homozygous for mip120LL07629 has greatly diminished Mip120 protein levels. hsFLP/+; FRT42B, mip120LL07629/FRT42B, Ubi-GFP-nls females.

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Fig. 10. A mutant clone homozygous for mip120LL07629 has greatly diminished Mip120 protein levels. hsFLP/+; FRT42B, mip120LL07629/FRT42B, Ubi-GFP-nls females were heat-shocked to induce the site-specific FLP recombinase, generating homozygous GFP-negative clones that were also homozygous for the mutant allele, mip120LL07629. A mutant clone homozygous for mip120LL07629 has greatly diminished Mip120 protein levels.hsFLP/+; FRT42B, mip120LL07629/FRT42B, Ubi-GFP-nls females were heat-shocked to induce the site-specific FLP recombinase, generating homozygous GFP-negative clones that were also homozygous for the mutant allele, mip120LL07629. Egg chambers were dissected, fixed, then stained with anti-Mip120 antibodies and TO-PRO-3. Follicle cells on the surface of the egg chamber were imaged by confocal microscopy for: GFP (top left); anti-Mip120 (top right); GFP (green) and anti-Mip120 (red) (bottom left); and TO-PRO-3 (bottom right). The boundary of the GFP-negative mutant clone is indicated by a dashed line. The TO-PRO-3 bright dots within the nuclei represent heterochromatin-dense chromocenters. Mei-Hsin Cheng et al. Biology Open 2017;6:967-978 © 2017. Published by The Company of Biologists Ltd