Filaggrin Degradation by Caspase-14 Is Required for UVB Photoprotection but Does Not Influence Allergic Sensitization in a Mouse Model of Atopic Dermatitis

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Filaggrin Degradation by Caspase-14 Is Required for UVB Photoprotection but Does Not Influence Allergic Sensitization in a Mouse Model of Atopic Dermatitis Michael Devos, Janne Prawitt, Delphine Staumont-Salle, Esther Hoste, Sebastién Fleury, Emmanuel Bouchaert, Barbara Gilbert, Saskia Lippens, Peter Vandenabeele, David Dombrowicz, Wim Declercq Journal of Investigative Dermatology Volume 132, Issue 12, Pages 2857-2860 (December 2012) DOI: 10.1038/jid.2012.236 Copyright © 2012 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Increased sensitivity to UVB-induced DNA damage in caspase-14-deficient epidermis is abrogated by topical trans-urocanic acid (trans-UCA) application. Trans-UCA (200μg) or vehicle (75% ethanol, 25% DMSO) was applied on ex vivo newborn back skin of wild-type and caspase-14-/- mice (10 generations backcrossed to BALB/c genetic background) before irradiation with 250mJcm-2 UVB (TL 20W/12 RS lamp, Philips, major UV emission in the 280–320nm range). One hour after irradiation, the tissue was snap-frozen and stored. DNA was prepared from the epidermis, and the concentration of cyclobutane pyrimidine dimers (CPDs) was determined by ELISA. The bars present the mean±SE. Values were compared using two-way analysis of variance; only significant differences are indicated, **P<0.01 (caspase-14+/+, vehicle: n=4, trans-UCA: n=8; caspase-14-/-, vehicle: n=5, trans-UCA: n=11). mOD, milli OD. Journal of Investigative Dermatology 2012 132, 2857-2860DOI: (10.1038/jid.2012.236) Copyright © 2012 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Similar ovalbumin (OVA)-specific immune response in caspase-14-deficient and wild-type mice. (a) May–Grünwald Giemsa staining of skin sections. (b) Epidermal thickness, and (c) total serum IgE levels. (d) Skin cytokine mRNA expression measured by quantitative reverse-transcription PCR: Th2 (IL-4 and IL-13), Th1 (IFN-γ), Th17 (IL-17), pro-inflammatory (IL-1β), and regulatory (IL-10) responses were evaluated. (e) Eosinophil density in dermis. Bars present the mean±SE. Values were compared using two-way analysis of variance; only significant differences are indicated, **P<0.01, ***P<0.001, (caspase-14+/+, phosphate-buffered saline: n=10, OVA: n=13; caspase-14-/-, phosphate-buffered saline: n=10, OVA: n=13); bar=50μm. Journal of Investigative Dermatology 2012 132, 2857-2860DOI: (10.1038/jid.2012.236) Copyright © 2012 The Society for Investigative Dermatology, Inc Terms and Conditions