Figure 1 BM community in the normozoospermic and iNOA human testis parenchyma. Quantification of the 16 S copies/ng in ... Figure 1 BM community in the.

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Figure 1 BM community in the normozoospermic and iNOA human testis parenchyma. Quantification of the 16 S copies/ng in ... Figure 1 BM community in the normozoospermic and iNOA human testis parenchyma. Quantification of the 16 S copies/ng in (i) 200 ng of total DNA from the PC3 cell line and 0.1 ng of total DNA from human buccal mucosa (BM), used as negative and positive controls, respectively and (ii) 200 ng of total DNA from normozoospermic and iNOA testis parenchyma (A). The microbial community in normozoospermic and iNOA testis parenchyma was analyzed for alpha and beta diversity. Diversity within samples (α-diversity) was estimated using >1200 sequences per sample (B, statistical significance by two-tail unpaired and non-parametric T-test). Diversity between samples (β-diversity) was evaluated by principal component analysis and represented by weighted variance (C). The relative distribution of taxonomic rank at the level of phyla (D) and class (E) for the BM of normozoospermic and iNOA testis parenchyma. Statistical significance was estimated by two-tail Mann–Whitney test and considered significant at a P-value <0.05 (represented by asterisks in panels D and E, when comparing normozoospermic vs. iNOA testis parenchyma). Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2018. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology.This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com Hum Reprod, Volume 33, Issue 7, 30 May 2018, Pages 1212–1217, https://doi.org/10.1093/humrep/dey116 The content of this slide may be subject to copyright: please see the slide notes for details.

Figure 2 BM community in the iNOA human testis parenchyma classified as sperm retrieval positive or germ cell aplasia. ... Figure 2 BM community in the iNOA human testis parenchyma classified as sperm retrieval positive or germ cell aplasia. Quantification of the 16 S copies/ng of loaded DNA in the (i) PC3 cell line and human buccal mucosa, BM, used as negative and positive controls, respectively, (ii) normozoospermic and iNOA testis parenchyma and (iii) the iNOA testis parenchyma according to positive sperm retrieval (R+) or complete germ cell aplasia (GCA) (A). The microbial community in iNOA-R+ and iNOA-GCA testis parenchyma was analyzed for alpha and beta diversity. Diversity within samples (α-diversity) was estimated using >1200 sequences per sample (B, statistical significance by two-tail unpaired and non-parametric T-test). Diversity between samples (β-diversity) was evaluated by principal component analysis and represented by weighted variance (C). The relative distribution of taxonomic rank at the level of phyla (D) and class (E) for the BM of iNOA-R+ and iNOA-GCA testis parenchyma. The relative distribution of the Clostridia taxa down to the species Peptoniphilus asaccharolyticus (F). Statistical significance was estimated by two-tail Mann–Whitney test and considered significant at a P-value <0.05 (represented by asterisks in panel E, when comparing iNOA-R+ vs. iNOA-GCA). Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2018. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology.This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com Hum Reprod, Volume 33, Issue 7, 30 May 2018, Pages 1212–1217, https://doi.org/10.1093/humrep/dey116 The content of this slide may be subject to copyright: please see the slide notes for details.