Fig. 4. Coilin phosphomutant displays differential RNA binding and degradation activities.Purified proteins were analyzed for RNase activity with total.

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Fig. 4. Coilin phosphomutant displays differential RNA binding and degradation activities.Purified proteins were analyzed for RNase activity with total RNA and RNA co-isolation amount. Coilin phosphomutant displays differential RNA binding and degradation activities.Purified proteins were analyzed for RNase activity with total RNA and RNA co-isolation amount. (A) Agarose gel with RNase reactions containing 500 ng HeLa RNA and the indicated amount of nucleic acid-bound coilin wild type (WT) or coilin S489D; lane 1 contains 1 kb+DNA ladder, lane 2 contains RNA alone, lanes 3 and 7 contain 500 ng of protein alone. (B) Agarose gel (top) showing co-purified nucleic acid of 1.5 µg nucleic acid-bound proteins and Coomassie stained SDS-PAGE gel (bottom) of 500 ng nucleic acid bound proteins. (C) Agarose gel with RNase reactions containing 500 ng HeLa RNA and the indicated amount of nucleic acid-free proteins; lane 1 contains 1 kb+DNA ladder, lane 2 contains RNA alone, lanes 3 and 7 contain 500 ng of protein alone. (D) Graph showing densitometric analysis of the 28S bands from C as a percent of the lane 2 control. Hanna J. Broome et al. Biology Open 2013;2:407-415 © 2013. Published by The Company of Biologists Ltd