Outcome of twin babies free of Von Hippel–Lindau disease after a double-factor preimplantation genetic diagnosis: monogenetic mutation analysis and comprehensive.

Slides:

Advertisements

Similar presentations

Mouse strain and quality control testing: improved sensitivity of the mouse embryo assay with embryos from outbred mice Zaraq Khan, M.B.B.S., Heather S.

Advertisements

What’s the delay? A qualitative study of women’s experiences of reaching a diagnosis of endometriosis Karen Ballard, Ph.D., Karen Lowton, Ph.D., Jeremy.

Pere Colls, Ph. D. , Tomas Escudero, M. Sc. , Natalie Cekleniak, M. D

Lynn B. Davis, M. D. , M. S. , Sara J. Champion, M. S. , Steve O

Susannah Baruch, J.D., David Kaufman, Ph.D., Kathy L. Hudson, Ph.D.

Multiple gestation associated with infertility therapy: an American Society for Reproductive Medicine Practice Committee opinion Fertility and Sterility

Elias M. Dahdouh, M. D. , M. Sc. , Jacques Balayla, M. D

Demián Glujovsky, M.D., M.Sc., Cynthia Farquhar, M.D., M.P.H.

Validation of microarray comparative genomic hybridization for comprehensive chromosome analysis of embryos Cristina Gutiérrez-Mateo, Ph.D., Pere Colls,

Distribution patterns of segmental aneuploidies in human blastocysts identified by next- generation sequencing María Vera-Rodríguez, M.Sc., Claude-Edouard.

Optimal euploid embryo transfer strategy, fresh versus frozen, after preimplantation genetic screening with next generation sequencing: a randomized controlled.

Evaluation of targeted next-generation sequencing–based preimplantation genetic diagnosis of monogenic disease Nathan R. Treff, Ph.D., Anastasia Fedick,

Lynn B. Davis, M. D. , M. S. , Sara J. Champion, M. S. , Steve O

Susannah Baruch, J.D., David Kaufman, Ph.D., Kathy L. Hudson, Ph.D.

A bovine protocol for training professionals in preimplantation genetic diagnosis using polymerase chain reaction Carlos Gilberto Almodin, M.D., Ph.D.,

Edward G. Hughes, M.B., F.R.C.S.(C.), Deirdre DeJean, M.Sc.

Modification of the triplet repeat primed polymerase chain reaction method for detection of the CTG repeat expansion in myotonic dystrophy type 1: application.

Development and validation of a next-generation sequencing–based protocol for 24- chromosome aneuploidy screening of embryos Francesco Fiorentino, Ph.D.,

Live birth after polar body array comparative genomic hybridization prediction of embryo ploidy—the future of IVF? Simon Fishel, Ph.D., Anthony Gordon,

Effect of a very-low-calorie diet on in vitro fertilization outcomes

Technology requirements for preimplantation genetic diagnosis to improve assisted reproduction outcomes Santiago Munné, Ph.D., Dagan Wells, Ph.D., Jacques.

Single-gene testing combined with single nucleotide polymorphism microarray preimplantation genetic diagnosis for aneuploidy: a novel approach in optimizing.

Pregnancy outcome after preimplantation genetic screening or natural conception in couples with unexplained recurrent miscarriage: a systematic review.

Can an educational DVD improve the acceptability of elective single embryo transfer? A randomized controlled study Nicole Hope, M.B., B.S., Hon., Luk.

The influence of female and male body mass index on live births after assisted reproductive technology treatment: a nationwide register-based cohort study

Preimplantation genetic testing: polar bodies, blastomeres, trophectoderm cells, or blastocoelic fluid? M. Cristina Magli, M.Sc., Alessandra Pomante,

Konstantinos Pantos, M. D. , Evangelos Makrakis, M. D

The relative contribution of assisted reproductive technologies and ovulation induction to multiple births in the United States 5 years after the Society.

Live birth of twins derived from zona-free oocytes

Development and validation of an accurate quantitative real-time polymerase chain reaction–based assay for human blastocyst comprehensive chromosomal.

Randomized, controlled pilot trial of natural versus hormone replacement therapy cycles in frozen embryo replacement in vitro fertilization Ginny Mounce,

Reproduction at an advanced maternal age and maternal health

Juliette Koch, M. R. A. N. Z. C. O. G. , Michael F. Costello, F. R. A

Economic evaluation of elective single-embryo transfer with subsequent single frozen embryo transfer in an in vitro fertilization/intracytoplasmic sperm.

Transvaginal versus transabdominal ultrasound guidance for embryo transfer in donor oocyte recipients: a randomized clinical trial Daniel Bodri, M.D.,

Agustín García-Peiró, B. S. , Juan Martínez-Heredia, Ph. D

Yinghui Ye, M. D. , Ph. D. , Yuqin Luo, B. Sc. , Yuli Qian, B. Sc

Vanessa L. Merker, B. S. , Timothy P. Murphy, J. Bryan Hughes, B. A

Xiuye Xing, M. D. , Ph. D. , Han Zhao, M. D. , Ph. D. , Mei Li, M. Sc

Application of three-dimensional fluorescence in situ hybridization to human preimplantation genetic diagnosis Li-Ying Yan, Ph.D., Jie Qiao, M.D., Yuan.

Single nucleotide polymorphism microarray–based concurrent screening of 24- chromosome aneuploidy and unbalanced translocations in preimplantation human.

Errors at mitotic segregation early in oogenesis and at first meiotic division in oocytes from donor females: Comparative genomic hybridization analyses.

Polar body biopsy Fertility and Sterility

Type of chromosome abnormality affects embryo morphology dynamics

Anne Girardet, Ph. D. , Céline Fernandez, B. Sc

Androgen insensitivity syndrome after preimplantation genetic diagnosis for sex selection: A case report Zaid Kilani, M.D., F.R.C.O.G., Yahia Dajani,

Anil Dubey, Ph. D. , Molina B. Dayal, M. D. , M. P. H

Akanksha Mehta, M.D., Darius A. Paduch, M.D., Ph.D.

Assessing the true incidence of mosaicism in preimplantation embryos

Cumulative newborn rates increase with the total number of transferred embryos according to an analysis of 15,792 ovum donation cycles Nicolás Garrido,

Dichorionic triamniotic triplet pregnancy with monozygotic twins discordant for trisomy 13 after preimplantation genetic screening: case report Deborah.

Accuracy of FISH analysis in predicting chromosomal status in patients undergoing preimplantation genetic diagnosis Catherine M. DeUgarte, M.D., Man.

Veronica Bertini, Ph. D. , Angelo Valetto, Ph. D. , Angela Uccelli, Ph

“Information-rich” reproductive outcomes in carriers of a structural chromosome rearrangement ascertained on the basis of recurrent pregnancy loss Michelle.

Cameron Gilbert, M. Sc. , Maria Valois, M. D. , FRCPC, Ph. D

Pregnancy outcome in infertile patients with polycystic ovary syndrome who were treated with metformin Samuel S. Thatcher, M.D., Ph.D., Elizabeth M.

Polymerase chain reaction-based detection of chromosomal imbalances on embryos: the evolution of preimplantation genetic diagnosis for chromosomal translocations

Comprehensive chromosome screening is highly predictive of the reproductive potential of human embryos: a prospective, blinded, nonselection study Richard.

Preimplantation genetic diagnosis for a carrier of a Y;autosome translocation resulting in a healthy male offspring Caroline Mackie Ogilvie, D.Phil.,

Genetic evaluation procedures at sperm banks in the United States

Blastocyst biopsy with comprehensive chromosome screening and fresh embryo transfer significantly increases in vitro fertilization implantation and delivery.

David H. Barad, M.D., M.S., Norbert Gleicher, M.D.

Filomenamila Lorusso, M. D. , Dewen Kong, M. D

What’s the delay? A qualitative study of women’s experiences of reaching a diagnosis of endometriosis Karen Ballard, Ph.D., Karen Lowton, Ph.D., Jeremy.

The use of arrays in preimplantation genetic diagnosis and screening

Chenming Xu, Ph. D. , Bingsen Xu, M. Sc. , Hefeng Huang, M. D

Randomized, controlled pilot trial of natural versus hormone replacement therapy cycles in frozen embryo replacement in vitro fertilization Ginny Mounce,

A novel Arg615Ser mutation of androgen receptor DNA-binding domain in three 46,XY sisters with complete androgen insensitivity syndrome and bilateral.

Preimplantation genetic screening: what is the clinical efficiency?

Not all twins are monozygotic after elective single embryo transfer: analysis of 32,600 elective single embryo transfer cycles as reported to the Society.

Presentation transcript:

Outcome of twin babies free of Von Hippel–Lindau disease after a double-factor preimplantation genetic diagnosis: monogenetic mutation analysis and comprehensive aneuploidy screening Albert Obradors, M.Sc., Esther Fernández, Ph.D., Mariona Rius, M.Sc., Maria Oliver-Bonet, Ph.D., Maria Martínez-Fresno, M.Sc., Jordi Benet, Ph.D., Joaquima Navarro, Ph.D. Fertility and Sterility Volume 91, Issue 3, Pages 933.e1-933.e7 (March 2009) DOI: 10.1016/j.fertnstert.2008.11.013 Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Aneuploid profiles observed for chromosomes #2, #10, #12, #17, #19, and #X of first polar body #1. Fertility and Sterility 2009 91, 933.e1-933.e7DOI: (10.1016/j.fertnstert.2008.11.013) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 2 The comparative genomic hybridization result for first polar body #8. Euploid profiles are observed from all analyzed chromosomes. The embryo that originated from it was transfered to the uterus, together with embryo #4, after being diagnosed as unaffected by VHL. Fertility and Sterility 2009 91, 933.e1-933.e7DOI: (10.1016/j.fertnstert.2008.11.013) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Electropherograms which resulted from the double-factor preimplantation genetic diagnosis of the biopsied embryos. (A) D3S1675 and D31537 short tandem repeat (STR) alleles from transfered embryo #8. (B) STR alleles from Von Hippel–Lindau syndrome (VHL)–affected embryo #6. (C) STRs from wild-type embryo #10, presenting allele dropout for allele 154 of D3S1675. (D) Minisequencing result for transfered embryo #8, showing a homozygote cytosine allele (black line). (E) Minisequencing result for VHL-affected embryo #6 with an allele for cytosine (black line) and the mutant one for thymine (red line). (F) Minisequencing result for VHL-affected embryo #10, presenting ADO for the wild-type allele (black line). Fertility and Sterility 2009 91, 933.e1-933.e7DOI: (10.1016/j.fertnstert.2008.11.013) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions