Protein Synthesis

POGIL REVIEW

Animation of the Flow of Information http://www.wiley.com/college/boyer/0470003790/animations/central_dogma/central_dogma.htm (skip to the end) Crick on central dogma http://www.dnalc.org/view/15473-The-Central-Dogma-transcription-and- translation-James-Watson.html

STICK PEOPLE DNA

TRANSCRIPTION

Animations of Transcription http://www.stolaf.edu/people/giannini/flashanimat/molgenetics/transcription.swf (simple and to the point) http://www-class.unl.edu/biochem/gp2/m_biology/animation/gene/gene_a2.html (little more complex) http://www.dnatube.com/video/5734/Central-dogma-of-transcription (virtual)

Open the DNA, read the template strand and follow the RNA base pairing rules… How Humans transcribe DNA Complementary Strand ATG TGG CGA mRNA AUG UGG CGA DNA Template Strand TAC ACC GCT

EDITTING Once mRNA is formed it is edited and sent out to the ribosome… The mRNA transcript has a cap and a tail added. The mRNA transcript has the region coding for introns removed. DNA is composed of both introns and exons. Exons are segments of DNA that are expressed as genes. Introns are ‘junk DNA.’ They are not expressed and must be removed before the mRNA leaves the nucleus.

Animation about mRNA editing http://www.dnatube.com/video/5930/MRna-Splicing-Video (splicing analogy with text)

TRANSLATION

Translation Animations http://www.stolaf.edu/people/giannini/flashanimat/molgenetics/translation.swf (simple and to the point) http://www-class.unl.edu/biochem/gp2/m_biology/animation/gene/gene_a3.html (little more complex) http://www.dnatube.com/video/5934/Basic-explanation-of-mRNA-Translation (virtual)

How humans translate Look up each mRNA codon on the table to determine the amino acid sequence. mRNA – AUG CCA GAG UAU UGA Amino acids –

Review of the flow of information Animation http://www.johnkyrk.com/er.html

Review Try it for yourself: http://www.pbs.org/wgbh/aso/tryit/dna/shockwave.html (replication & protein synthesis) Very detailed 10 minute video of protein synthesis www.youtube.com/watch?v=J3HVVi2k2No

BINGO with the genetic code Remember – This is mRNA AUG = start codon

Function/Purpose of the structure Structure letter Name of the structure Function/Purpose of the structure A   B C D E F