Directed differentiation of hPSCs.

Slides:

Advertisements

Similar presentations

GROWTH FACTOR SIGNALING, HOX GENES AND THE BODY PLAN

Advertisements

Stem Cells and Cell Signaling

Current Model for how cells become neural 1)Default state is neural 2)Local secretion of BMPs by epidermis inhibits neural fate 3)Local secretion of noggin,

I. Ectoderm. 60 X 1.Neural tube3. somite 5. yolk sac 7. aorta 2.Amniotic cavity 4. nephrogenic cord 6. notochord8. embryonic.

Axis determination and early development in amphibians

A CRISPR/Cas-Mediated Selection-free Knockin Strategy in Human Embryonic Stem Cells Zengrong Zhu, Nipun Verma, Federico González, Zhong-Dong Shi, Danwei.

From Stem Cells to Beta Cells: Possible Cure for Diabetes Mellitus By Ryan Scavinski.

What is differentiation of pluripotent cells?

References  Lecture notes (hyperlink)  Activity notes (hyperlink)  More links… Directed differentiation of ES cells into ectoderm.

Smad7-induced b-catenin degradation alters epidermal appendage development Han, G. et al., 2006, Dev Cell.

Axis determination and early development in amphibians

Axis determination and early development in amphibians

Shin-Ichi Nishikawa, MD, PhD

Research, 2016, Vol. 4, No. 1, doi: /jfnr-4-1-6

Stem Cells Key Words: Embryonic stem cells, Adult stem cells, iPS cells, self-renewal, differentiation, pluripotent, multipotent, Inner cell mass, Nuclear.

Translation: Screening for Novel Therapeutics With Disease-Relevant Cell Types Derived from Human Stem Cell Models Stephen J. Haggarty, Roy H. Perlis

Figure 2 Crosstalk between TGF-β/Smad and other pathways in tissue fibrosis Figure 2 | Crosstalk between TGF-β/Smad and other pathways in tissue fibrosis.

Inducing Angiogenesis

Axis determination and early development in amphibians

Pocket-sized iron regulators: one size fits all?

Figure 2 Functional model of enthesitis

Figure 3 Extracellular stimuli to HSC activation

How do cells talk to each other

Functional neutrophils from human ES cells

Kristopher G. Maier, PhD, Xuan Han, Benjamin Sadowitz, MD, Karen L

Management of advanced renal cancer

Figure 1 The central role of chronic prostate

by Yuka Nagata, Eisuke Nishida, and Kazuo Todokoro

From Embryonic Stem Cells to β-Cells: New Hope for Diabetics?

Cerebral Organoids in a Dish: Progress and Prospects

Production of De Novo Cardiomyocytes: Human Pluripotent Stem Cell Differentiation and Direct Reprogramming Paul W. Burridge, Gordon Keller, Joseph D.

Local and systemic factors and implantation: what is the evidence?

Human Induced Pluripotent Stem Cell–Derived Ectodermal Precursor Cells Contribute to Hair Follicle Morphogenesis In Vivo Ophelia Veraitch, Tetsuro Kobayashi,

NRG4: An Endocrine Link between Brown Adipose Tissue and Liver

Steve S.W. Han, Luis A. Williams, Kevin C. Eggan Neuron

Volume 4, Issue 4, Pages (April 2015)

Volume 9, Issue 3, Pages (September 2017)

Volume 2, Issue 3, Pages (March 2014)

A Breath of Fresh Air in Lung Regeneration

Daria Prilutsky, Nathan P

Figure 2 Neuron differentiation and validation

Retinoic acid sentinels in live embryos and responding to exogenous RA in half-embryo cultures. Retinoic acid sentinels in live embryos and responding.

Fig. 2. Morphological changes of cultured adherent fibroblastic cells after OA treatment related to actin microfilament reorganization.(A) Cells observed.

Post-ischemic vascular repair mechanisms and the growth factors involved. Post-ischemic vascular repair mechanisms and the growth factors involved. The.

In vivo location and in vitro generation of hypothalamic neurons.

Fig. 3. Inactivation of the Wnt/β-catenin signaling pathway inhibited cell proliferation and induced apoptosis in A549 and SPC-A-1 cells. Inactivation.

Established and putative mediators and pathways involved in the pathogenesis of pulmonary arterial hypertension. Established and putative mediators and.

Preformation and induction models of PGC specification.

Dose-responsiveness and specificity of retinoic acid signaling sentinels. Dose-responsiveness and specificity of retinoic acid signaling sentinels. (A)

Fig. 2. Blocking of BMP signaling, but not of nodal signaling, inhibits autochthonous neural crest migration in zebrafish embryos. Blocking of BMP signaling,

Hypothetical model of HvAP2 control of stem elongation.

Inhibition of VEGF-R2-mediated signalling cascade by endogenous antagonists. Inhibition of VEGF-R2-mediated signalling cascade by endogenous antagonists.

The derivation of hESCs and hiPSCs.

Fig. 6. Apical polarity of primitive endoderm cells on surface of embryoid bodies.Embryoid bodies were analyzed by immunofluorescence microscopy for GATA4.

Fig. 4. SMXL6 is degraded in response to SL treatment.

Model of the Ang-1 pathway in early lung development.

Volume 9, Issue 4, Pages (October 2017)

Integrating and converting biophysical signals.

Niels Grote Beverborg et al. JCHF 2018;6:

Molecular pathways underlying angiogenesis.

Self-organization into organoids, gastruloids and embryoids.

Fig. 3. Effects of Tec on IL-1β-induced apoptosis in chondrocytes.

Signalling specificity in the TGF-β superfamily.

Fig. 1. Cell adhesion molecule expression and the aggregation of wildtype and mutant ES cells.(A) Wildtype (WT), E-cadherin null (9J), and N-cadherin null.

Directing Lung Endoderm Differentiation in Pluripotent Stem Cells

Compounds acting on pathogenic pathways of pulmonary arterial hypertension. Compounds acting on pathogenic pathways of pulmonary arterial hypertension.

Nat. Rev. Endocrinol. doi: /nrendo

Overview of important signalling pathways in angiogenesis and antiangiogenic agents. Overview of important signalling pathways in angiogenesis and antiangiogenic.

Pathogenic concepts of pulmonary arterial hypertension (PAH).

Kidney Organoids: A Translational Journey

Presentation transcript:

Directed differentiation of hPSCs. Directed differentiation of hPSCs.In vitro differentiation of human pluripotent stem cells (hPSCs) can be performed in adherent culture or in suspension culture via embryoid body (EB) formation. In both formats, differentiation can be induced by treatment with growth factors and small molecules to activate or inhibit various signaling pathways in a step-wise manner by mimicking embryonic development. Typical differentiation protocols are illustrated using three specific examples: motoneurons from the ectoderm (Li et al., 2005; Wichterle et al., 2002), erythropoietic cells from the mesoderm (Niwa et al., 2011) and intestinal cells from the endoderm (Spence et al., 2011). In each case, the signaling factors and pathways required (or those that need to be inhibited) to drive differentiation into the appropriate cell types are indicated. BMP, bone morphogenetic protein; EGF, epidermal growth factor; EPO, erythropoietin; FGF, fibroblast growth factor; FP6, interleukin 6 (IL6) and IL6 receptor fusion protein; IL, interleukin; RA, retinoic acid; SCF, Kit ligand; SHH, sonic hedgehog; TPO, thyroid peroxidase; VEGF, vascular endothelial growth factor. Zengrong Zhu, and Danwei Huangfu Development 2013;140:705-717 © 2013. Published by The Company of Biologists Ltd