Steps needed to set up a modern microscope Steps needed to set up a modern microscope.Switch on light sourcePlace slide on stage with the sample in the light pathSelect a low power objective (x 4)Focus on sample to allow further adjustmentsAdjust the interpupillary distance and the eyepiece for any differences between your eyesTo focus the condenser for optimum resolution, a sharp object placed centrally at the light source or alternatively the edge of the closed light source diaphragm (if present) is made sharp by altering the height of the condenser. Steps needed to set up a modern microscope.Switch on light sourcePlace slide on stage with the sample in the light pathSelect a low power objective (x 4)Focus on sample to allow further adjustmentsAdjust the interpupillary distance and the eyepiece for any differences between your eyesTo focus the condenser for optimum resolution, a sharp object placed centrally at the light source or alternatively the edge of the closed light source diaphragm (if present) is made sharp by altering the height of the condenser. This is usually a high position, see Figure 4Adjust the condenser diaphragm to the correct position for the lens you are usingMinimise scatter by adjusting the light source diaphragm to the minimum diaphragm needed (not available on all microscopes)‏ Stephen Shaw In Practice 2016;38:12-16 Copyright © British Veterinary Association. All rights reserved.