The glutathione-S-transferase Mu 1 null genotype modulates ozone-induced airway inflammation in human subjects Neil E. Alexis, PhD, Haibo Zhou, PhD,

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The glutathione-S-transferase Mu 1 null genotype modulates ozone-induced airway inflammation in human subjects Neil E. Alexis, PhD, Haibo Zhou, PhD, John C. Lay, PhD, Bradford Harris, MD, Michelle L. Hernandez, MD, Tsui-Shan Lu, PhD, Philip A. Bromberg, MD, David Diaz-Sanchez, PhD, Robert B. Devlin, PhD, Steven R. Kleeberger, PhD, David B. Peden, MD Journal of Allergy and Clinical Immunology Volume 124, Issue 6, Pages 1222-1228.e5 (December 2009) DOI: 10.1016/j.jaci.2009.07.036 Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Effect of GSTM1 genotypes on lung function response to ozone: FEV1(top) and FVC (bottom) expressed as mean (SEM) percentages of baseline values 4 and 24 hours after ozone exposure in GSTM1+ (n = 19, solid bars) and GSTM1− (n = 16, open bars) subjects. Journal of Allergy and Clinical Immunology 2009 124, 1222-1228.e5DOI: (10.1016/j.jaci.2009.07.036) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Effect of GSTM1 genotypes on PMN and macrophage numbers in sputum in response to ozone: mean (SEM) PMNs per milligram of sputum (top) and macrophages per milligram of sputum (bottom) before (baseline) and 4 and 24 hours after ozone exposure in GSTM1+ (n = 19, solid bars) and GSTM1− (n = 16, open bars) subjects. ∗P < .05 versus baseline. +P < .05, GSTM1+ versus GSTM1−. Journal of Allergy and Clinical Immunology 2009 124, 1222-1228.e5DOI: (10.1016/j.jaci.2009.07.036) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Effect of GSTM1 genotypes on cell function 24 hours after ozone exposure: mean (SEM) oxidative burst activity MFI (top, macrophages, GSTM1+ n = 17, GSTM1− n = 16), phagocytosis (middle, macrophages, GSTM1+ n = 19, GSTM1− n = 15), and HLA-DR expression MFI (bottom, GSTM1+ n = 9, GSTM1− n = 11) at 4 and 24 hours after ozone exposure in GSTM1+ and GSTM1− subjects. ∗P < 0.05 versus before. +P < .05 for 24-hour response in GSTM1+ versus GSTM1− subjects. Journal of Allergy and Clinical Immunology 2009 124, 1222-1228.e5DOI: (10.1016/j.jaci.2009.07.036) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Effect ofGSTM1 genotypes on CD14 expression on airway PMNs 24 hours after ozone exposure. ∗P = .002 for the GSTM1− cohort comparing baseline with 24-hour values within this genotype. +P = .05 change from baseline values at 24 hours in GSTM1− versus GSTM1+ volunteers. Journal of Allergy and Clinical Immunology 2009 124, 1222-1228.e5DOI: (10.1016/j.jaci.2009.07.036) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Effect ofGSTM1 genotypes on IL-1β and IL-8 levels in airway sputum recovered after exposure to ozone: GSTM1− volunteers had a significant increase in IL-1β levels 4 and 24 hours after completion of the ozone exposure protocol, as well as IL-8 levels 24 hours after ozone exposure. Nominal P values are noted in the body of the figure. Journal of Allergy and Clinical Immunology 2009 124, 1222-1228.e5DOI: (10.1016/j.jaci.2009.07.036) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions