The interaction of SYCE2 and HP1α is not modified in response to exogenous DNA damage induced by x-ray irradiation. The interaction of SYCE2 and HP1α is not modified in response to exogenous DNA damage induced by x-ray irradiation. (A) Interaction of FLAG-SYCE2 with HP1α in FLAG-SYCE2–expressing RPE cells unirradiated or irradiated with 8-Gy x-ray 1 h before harvesting the cell lysates. 500 μg of total cell lysates was immunoprecipitated using the anti-FLAG antibody or normal mouse IgG as a negative control and visualized by Western blotting using the anti-HP1α antibody (upper panel). Noriko Hosoya et al. LSA 2018;1:e201800021 © 2018 Hosoya et al.