Fig. 7. Ror-GFP binds to Myc-tagged Wnts and Wnt receptors.

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Suppl.Figure 1 Transfected HEK293 cells -130kDa -100KDa Blot: -V5

Volume 15, Issue 22, Pages (November 2005)

Supplement Figure 2 HEK 293 cells

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Fig. 7. Ror-GFP binds to Myc-tagged Wnts and Wnt receptors. Ror-GFP binds to Myc-tagged Wnts and Wnt receptors. (A) The indicated constructs for Ror-GFP, CD8-GFP as negative control and Myc-tagged Wnts were transfected into Drosophila S2R+ cells. Co-immunoprecipitation from cell lysates was performed using mouse anti-GFP antibody followed by western blotting using mouse anti-Myc and rabbit anti-GFP antibodies. In the blot probed with anti-Myc antibody the denatured heavy chain of the immunoglobulin used in the IP is visible (marked with °). Bands representing the respective full-length proteins are marked with asterisks (*). (B) The indicated constructs for Ror-GFP, CD8-GFP as negative control and Myc-tagged Wnt receptors were co-transfected into Drosophila S2R+ cells. Co-immunoprecipitation from cell lysates was performed as above. IP, immunoprecipitation; Blot, western blot. Protein sizes are indicated in kilo Dalton (kD). Caroline Ripp et al. Biology Open 2018;7:bio033001 © 2018. Published by The Company of Biologists Ltd