All Hematopoietic Stem Cells Engraft in Submyeloablatively Irradiated Mice Katarina Forgacova, Filipp Savvulidi, Ludek Sefc, Jana Linhartova, Emanuel.

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All Hematopoietic Stem Cells Engraft in Submyeloablatively Irradiated Mice Katarina Forgacova, Filipp Savvulidi, Ludek Sefc, Jana Linhartova, Emanuel Necas Biology of Blood and Marrow Transplantation Volume 19, Issue 5, Pages 713-719 (May 2013) DOI: 10.1016/j.bbmt.2013.02.012 Copyright © 2013 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 1 Survival of STRCs and LTRCs after irradiation with various doses. (A) Experimental data. (B) Derivation of the proportional survival estimates. Data are presented as mean ± SE of the mean (n = 3). Regression analysis: nonlinear regression using the log (inhibitor) versus response curve – variable slope (4 parameters) calculation. Biology of Blood and Marrow Transplantation 2013 19, 713-719DOI: (10.1016/j.bbmt.2013.02.012) Copyright © 2013 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 2 Schematic representation of experiments for determining dependence of donor chimerism on submyeloablative conditioning irradiation of host congenic mice. *Average from estimates derived from chimerism determined in blood and bone marrow (see Figure 1B). Biology of Blood and Marrow Transplantation 2013 19, 713-719DOI: (10.1016/j.bbmt.2013.02.012) Copyright © 2013 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 3 Donor chimerism determined after 1 month (STRCs) in peripheral blood (PB) and after either 4 (A, B) or 6 (C, D) months (LTRCs) in peripheral blood and bone marrow (BM) in progressively irradiated recipients transplanted with a standard dose of normal bone marrow cells (see Figure 2). Data are presented as mean ± SE of the mean (n = 5). Regression analysis: nonlinear regression using the log (inhibitor) versus response curve – variable slope (4 parameters) calculation. Biology of Blood and Marrow Transplantation 2013 19, 713-719DOI: (10.1016/j.bbmt.2013.02.012) Copyright © 2013 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 4 Pooled results from the 4 separate experiments presented in Figure 3 (empty circles and solid curve) plotted against curves showing the theoretically expected chimerism corresponding to 100%, 80%, 60%, 40%, and 20% engraftment efficiency derived from calculated data presented in Table S1 (dashed curves). Biology of Blood and Marrow Transplantation 2013 19, 713-719DOI: (10.1016/j.bbmt.2013.02.012) Copyright © 2013 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 5 Pooled results from the 4 separate experiments presented in Figure 3 (empty circles and solid curve) plotted against curves showing the theoretically expected chimerism corresponding to 100% engraftment efficiency and the bone marrow from a one-half femur representing from 2.5% to 4.5% of total marrow cells. Biology of Blood and Marrow Transplantation 2013 19, 713-719DOI: (10.1016/j.bbmt.2013.02.012) Copyright © 2013 American Society for Blood and Marrow Transplantation Terms and Conditions