Fig. 1. TSA at 165 nM induces maximal acetylation of histone H3 and near-maximal acetylation of histone H4.Immunoblot analysis of acetylated histones H3.

Slides:

Advertisements

Similar presentations

NM-3 induces p21 levels and down-regulation of Cdk2 activity.

Advertisements

Fig. 8. CCA and ChQ treatment induce accumulation of F-actin rings.

VacA does not induce phosphorylation of Src in RPTPα constitutive-knockdown AZ-521 cells. VacA does not induce phosphorylation of Src in RPTPα constitutive-knockdown.

Fig. 1. Chlamydia infection causes elevated levels of sortilin.

Fig. 2. Morphological changes of cultured adherent fibroblastic cells after OA treatment related to actin microfilament reorganization.(A) Cells observed.

Fig. 7. Motion adaptation increases time-dependent response modulations (TDRM) relatively to the average cell response.TDRM normalized to the value obtained.

The histidine-rich loop regulates accessibility of the active site and RDEL motif in vivo. The histidine-rich loop regulates accessibility of the active.

Fig. 1. Muscle-specific PITX1 over-expression in the Pitx1 transgenic mice.(A) Detection of Pitx1 mRNA expression in muscles of the Pitx1 transgenic mice.

Fig. 4. A primary screen based on scrape closure

Fig. 1. Blood lactate, blood glucose and blood corticosterone concentration from crawl until 4 hours of frenzy swimming in loggerhead (C. caretta) and.

Fig. 5. Expression of either dFMRP or human FMRP does not induce eIF2α phosphorylation.(A) Analysis of eIF2α phosphorylation upon dFMRP expression. Expression.

TP53 western blot of primary cultured tail cells from both wild-type (WT) and Tp53Δ11/Δ11 (−/−) rats. TP53 western blot of primary cultured tail cells.

Fig. 1. Lack of Hmga1 and Hmga2 expression in A1/A2-KO mice

Fig. 4. The model of malate metabolism in fruit cells under different K level conditions. The model of malate metabolism in fruit cells under different.

Fig. 3. Relative expression levels of ASNS to α-tubulin were dramatically increased when treating human cells with nocodazole and ASNase. Relative expression.

Fig. 7. E2F1 acetylation in A1/A2-KO MEFs

Fig. 4. Brood size of four successive births by male seahorses Hippocampus erectus in the two groups (TR-1, TR-2).In TR-1 groups: male and female seahorses.

Elevated pAkt level in ptena−/−ptenb−/− mutants is suppressed by LY and to a lesser extent by sunitinib. Elevated pAkt level in ptena−/−ptenb−/−

Dose-responsiveness and specificity of retinoic acid signaling sentinels. Dose-responsiveness and specificity of retinoic acid signaling sentinels. (A)

Fig. 5. Morphological changes of the N

Fig. 7. ML mediated suppression of SAHA-induced up-regulation of tRNA as a potential mechanism that contributes to the enhanced anti-cancer actions.

Fig. 4. Relative expression levels of glycogen phosphorylase (GP), carnitine palmitoyltransferase I (cpt 1), glycogen synthase and acetyl CoA carboxylase.

Fig. 6. Effect of SAHA and ML on histone acetylation, BAX, and p21CDKN1A expression.PANC-1 and BxPC-3 cells were incubated for 48 hours with 5 µM.

Fig. 2. Blocking of BMP signaling, but not of nodal signaling, inhibits autochthonous neural crest migration in zebrafish embryos. Blocking of BMP signaling,

Fig. 5. Receptor tyrosine kinase activation in response to growth factor stimulation. Receptor tyrosine kinase activation in response to growth factor.

FAK tyrosine phosphorylation is unaffected by loss of LAR activity.

Fig. 2. Centrosomal proteins display distinct localizations and radial distances from centriole walls.U2OS cells were fixed and stained with the indicated.

Fig. 6. Coilin mutants display differential hTR processing activity but lack specificity toward the 5′ or 3′ regions.(A) Illustration of human telomerase.

Fig. 4. One-dimensional gel electrophoresis of DIMs

Fig. 2. Pull down of CaV2. 2 by channel C terminal fusion protein

Fig. 3. Rnd proteins induce stronger responses in subconfluent endothelial cells.HUVECs were transfected with Rnd1, Rnd2, Rnd3 or GFP-encoding plasmids.

Fig. 2. Soluble sugar and organic acid levels with different K fertilization during fruit development. Soluble sugar and organic acid levels with different.

Fig. 1. Aboveground biomass of Caragana and herbaceous plants, and proportional abundance of Caragana, under different grazing management treatments. Aboveground.

Fig. 4. Coilin phosphomutant displays differential RNA binding and degradation activities.Purified proteins were analyzed for RNase activity with total.

Fig. 3. Reduced histone H3K9 trimethylation in SCNT embryos treated with TSA and VC. (A) Immunostaining analysis indicated that SCNT embryos with TSA+VC.

Fig. 5. Combination of SAHA and ML produces augmented suppression of cellular proliferation with impaired cell cycle progression, enhanced apoptotic.

Fig. 2. TSA impairs growth and disrupts morphogenesis of exocrine pancreas in zebrafish larvae with hyperacetylation of nucleosomal histones.WT zebrafish.

Therapy-induced senescence of primary cells.

Fig. 4. Quantitative mRNA expression of two membrane-bound trehalase genes in Harmonia axyridis in response to starvation (0–72 h). Quantitative mRNA expression.

Fig. 4. Spectra of monochromatic light from the OLS

Oral administration of CX-4945 suppresses Tau phosphorylation in DYRK1A-overexpressing mice. Oral administration of CX-4945 suppresses Tau phosphorylation.

Fig. 3. Enhanced EGFR signaling in Rhbdf2P159L/P159L mice.

Folic acid increases apical junctional pMLCK localization in vivo

The SIM domain of RAD51 is required for HR

Fig. 4. Impaired induction of TH-response genes in trβ crispants

Fig. 3. Effects of Tec on IL-1β-induced apoptosis in chondrocytes.

Lysine residues in the cytoplasmic region of TfR are involved in the MARCH8-induced downregulation of TfR. Lysine residues in the cytoplasmic region of.

Effect of the siRNA-mediated knockdown of endogenous MARCH8 on the expression levels of MARCH8 substrates, TfR and CD98, in HepG2 cells. Effect of the.

Morphological changes induced by T3 treatment in trβ crispants

The zebrafish swdp75fm and swdp82mf mutations cause exocrine pancreatic hypoplasia and reduced skin pigmentation. The zebrafish swdp75fm and swdp82mf mutations.

Fig. 5. Coilin mutants display differential U2 processing activity with specificity toward the CU region.Purified proteins were incubated with in vitro.

Fig. 2. Acetylation stiffens primary cilia.

Fig. 1. Cell adhesion molecule expression and the aggregation of wildtype and mutant ES cells.(A) Wildtype (WT), E-cadherin null (9J), and N-cadherin null.

Fig. 5. Testis defects in STK35 KO mice.

Fig. 1. p85β localizes at adhesion plaques and associates with FAK

Fig. 5. Upregulation and subcellular relocalization of Hsp70 in striated muscles overexpressing either DVAP-V260I or DVAP-WT constructs.(A) NMJs of BG57-Gal4/+

Fig. 3. Mean force and velocity during jumping

Table 1. Measurement of ring diameters of proteins localizing in ring-like patterns around centrioles.Consideration of the size of IgG (about 8 nm) raises.

WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates TfR. WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates.

SpC3 concentrations in coelomic fluid (CF) collected from sea urchins at various times before and after the injection of LPS or ASW. Data are shown as.

Fig. 3. Changes in the total EPS/Chl a ratio and bend interval of trichomes before and after the removal of polysaccharide from the BG11-cultured N. flagelliforme.

Effects of paclitaxel and vinblastine.

Ub-binding ability of ERα.

Expression of IP3R subtypes in HEK cells.

BEZ235 induces MAPK signaling in a FOXO3A-dependent manner.

Inhibition of macrophage STAT3 activation during ADPKD programming blunts development of the pathological pro-proliferative macrophage phenotype. Inhibition.

Maytansinol disrupts the mitotic spindle and prevents mitotic exit in Drosophila. Maytansinol disrupts the mitotic spindle and prevents mitotic exit inDrosophila.

Regulation of Bcl-2 family proteins in the hypothalamus of non-diabetic and diabetic IRS2−/− mice. Regulation of Bcl-2 family proteins in the hypothalamus.

Effect of SFN on the protein expression of Nrf2, HO-1, and NQO1 in JB6-shMock and JB6-shNrf2 cells. Effect of SFN on the protein expression of Nrf2, HO-1,

Effect of SFN on the total activity and protein expression of HDACs in JB6 P+ cells. Effect of SFN on the total activity and protein expression of HDACs.

Presentation transcript:

Fig. 1. TSA at 165 nM induces maximal acetylation of histone H3 and near-maximal acetylation of histone H4.Immunoblot analysis of acetylated histones H3 and H4. TSA at 165 nM induces maximal acetylation of histone H3 and near-maximal acetylation of histone H4.Immunoblot analysis of acetylated histones H3 and H4. WT zebrafish larvae at 48 h.p.f. were incubated with TSA at various concentrations, DMSO, or no treatment, for 24 hours. Lane 1 (8.25 nM TSA), 2 (16.5 nM TSA), 3 (41.25 nM TSA), 4 (82.5 nM TSA), 5 (165 nM TSA), 6 (330 nM TSA), 7 (825 nM TSA), 8 (0.5% DMSO), and 9 (no treatment). Total protein was extracted from each group of larvae at 72 h.p.f., and analyzed by immunoblotting using the indicated antibodies. The intensity and area of each protein band was quantified by densitometric analysis. Each value represents the ratio of acetylated histone H3 (AcH3) to total histone H3, and acetylated histone H4 (AcH4) to H4, relative to that of control (no treatment). Nelson S. Yee et al. Biology Open 2012;1:295-307 © 2012. Published by The Company of Biologists Ltd