Fig. 1. Mitotic arrest results in differential RNA association with coilin.Untreated or nocodazole treated HeLa cell lysate was used for RNA immunoprecipitations.

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Fig. 1. Mitotic arrest results in differential RNA association with coilin.Untreated or nocodazole treated HeLa cell lysate was used for RNA immunoprecipitations with rabbit IgG and α-coilin antibodies. Mitotic arrest results in differential RNA association with coilin.Untreated or nocodazole treated HeLa cell lysate was used for RNA immunoprecipitations with rabbit IgG and α-coilin antibodies. RNA isolated from the IP complexes was analyzed by qRT-PCR with specific primers. Histogram of qRT-PCR data from RNA IP with IgG or α-coilin in untreated or nocodazole treated HeLa cells; fold enrichment of α-coilin IP over IgG control is shown on a log10 scale; specific primer target RNA is noted beneath each column pair; error bars represent 1 s.d. (data derived from at least 3 independent experiments with at least 2 technical repeats per experiment); *P<0.02 relative to GAPDH within a treatment; #P<4E−4 relative to untreated. The data for the untreated samples have been reported elsewhere (Broome and Hebert, 2013). Hanna J. Broome et al. Biology Open 2013;2:407-415 © 2013. Published by The Company of Biologists Ltd