Detection of nuclear foci by FISH on frozen sections of diaphragm muscle prepared from 2-month-old DMSXL mice. Detection of nuclear foci by FISH on frozen.

Slides:

Advertisements

Similar presentations

A B C D % positive cells 10 % Apoptotic nuclei 10 Figure S5 PM

Advertisements

Figure S1 a b c d DAPI Lgr β-catenin Merge

TMZ and Ara-C treatment of slice co-cultures.

Osteosarcoma histology.

Detection and tracking of individual SNAP-tagged proteins on the surface of living cells. Detection and tracking of individual SNAP-tagged proteins on.

The pathophysiological processes linked to kidney disease.

Distinct collagen structures in the upper and lower neonatal dermis (related to Fig 1)‏ Distinct collagen structures in the upper and lower neonatal dermis.

Percentage fractions of (β-Gal+) PCs in 32-week-old heterozygous females differ between cerebellar lobules affected and spared from degeneration of Purkinje.

ILK knockdown decreases mTOR signaling in PKD kidneys.

Fig. 1. Overview of the nervous system of the adult S. roscoffensis.

Fig. 8. In vitro effect of CMPs on adult DSS-treated stomach explants.

TSA causes premature chromatid sister separation.

Fig. 1. Representative images of the four cell lines using fluorescence microscopy. Representative images of the four cell lines using fluorescence microscopy.

Representative illustrations of a sagittal lung section stained with H&E in a control and a CS-exposed mouse after 6 months. Representative illustrations.

Nephrogenesis in vitro can be rescued in 3D co-cultures by siRNA treatment of Renca cells. Nephrogenesis in vitro can be rescued in 3D co-cultures by siRNA.

Fig. 6. Hts regulates par-1 and camkII mRNA distribution and levels in the muscle.(A–J) Views of muscles 6/7 in abdominal segment 4, probed for either.

Localisation of epithelial markers in mMEC cultures.

Fig. 6. Cross-section of the stomach wall and spiral intestine of the embryo, stained with PAS. (A) Surface of the stomach wall (SW) and ingested material.

OA is found in the central and peripheral nervous systems.

Tomoregulin-1 improves cardiac pathological changes after pressure overload. Tomoregulin-1 improves cardiac pathological changes after pressure overload.

OCT and IOP analysis of 2-3-month-old AP-2β NCC KO mutants.

Embryonic corneal defects in E18.5 AP-2β NCC KO mutant embryos.

Inflammation is associated with increased basal-cell plasticity in the Nkx3.1 mutant prostate. Inflammation is associated with increased basal-cell plasticity.

Fig. 1. Mitochondrial internalization in cardiomyocytes.

Expression of tomoregulin-1 in wild-type and cardiac hypertrophy mouse myocardium. Expression of tomoregulin-1 in wild-type and cardiac hypertrophy mouse.

Challenge with oxazolone results in epithelial hyperplasia and influx of inflammatory cells into the epidermis and dermis and keratosis as observed in.

Secondary storage of GM2 ganglioside and cholesterol in Fuca1-deficient mice. Secondary storage of GM2 ganglioside and cholesterol in Fuca1-deficient mice.

Latency to onset of Stage III behavioral seizure activity.

The initial targeting of Sec61b mRNA to the ER is partially dependent on ribosomes and translation. The initial targeting of Sec61b mRNA to the ER is partially.

Fig. 4. Detection of dFMR1 mRNA in dFMRP granules by FISH

Rescue of motoneuron degeneration by small molecules in ATXN3-CAG89 transgenics. Rescue ofmotoneurondegeneration by small molecules in ATXN3-CAG89 transgenics.

Formation of papilloma lesions in the UroIICre+Fgfr3+/K644EK-RasG12D/+ model. Formation of papilloma lesions in theUroIICre+Fgfr3+/K644EK-RasG12D/+model.

Fgfr3 mutation is not the sole driver of tumorigenesis in the bladder.

Increased osteoclastogenesis in the mandibular bone of Fgfr2+/S252W embryos at E16.5. Increased osteoclastogenesis in the mandibular bone of Fgfr2+/S252W.

Α-Catenin DKO cardiomyocytes exhibit increase proliferation on stiff ECM. (A) Representative images of neonatal cardiomyocytes cultured on different PA-FN.

Fig. 7. Representative images of control (Cas9+GFP) and Cas9+gRNA+GFP co-injected embryos on day 4 of culture, showing nuclear-imported GFP (green) and.

Morphological changes in skeletal muscles during aging in mammals.

Fig. 4. CIZ1 reduces the impact of injury to the heart.

Characterization of zebrafish muscle wasting in krasG12V-induced HCC zebrafish. 4-month-old male adult krasG12V and WT zebrafish were treated with dox.

Cellular reaction to intraventricular DMSO injection.

Fig. 2. Sufficient rate of recombination in embryonic NSCs and NPCs of Emx1cre knock-in mice.(A) Breeding schemes to generate mice with the Emx1cre locus.

Rgs16::GFP expression in pancreatic neoplasia.

Impaired elastogenesis in Ltbp4S−/−;Fibulin-4R/R mice with reduced interaction of Ltbp-4L and fibulin-4. Impaired elastogenesis in Ltbp4S−/−;Fibulin-4R/R.

Expression analysis of periostin in DRG

Sox2 regulates dental epithelial stem cell proliferation and differentiation. Sox2 regulates dental epithelial stem cell proliferation and differentiation.

Tumor location determines response to intravenous FOLFIRINOX chemotherapy. Tumor location determines response to intravenous FOLFIRINOX chemotherapy. (A)

Nerve conduction slowing and DRG sensory neuron and nuclear atrophy in 16-week-old diabetic mice. Nerve conduction slowing and DRG sensory neuron and nuclear.

Z-stack compressions showing triple labelling of different cell types in the gills of bowfin with antibodies for serotonin (5-HT, green) and HNK-1 (magenta),

Exo70 is recruited to the plasma membrane at sites of mechanical wounding. Exo70 is recruited to the plasma membrane at sites of mechanical wounding. NRK.

Fgfr3;4 mutant lungs display an increase in Mfap5, Igf1 and Fbn2 expression. Fgfr3;4 mutant lungs display an increase in Mfap5,Igf1and Fbn2 expression.

Histological analyses for limb-specific Uhrf1 KO mice.

Pgc-1α+/− mice show an increased inflammatory response to LPS injection. Pgc-1α+/− mice show an increased inflammatory response to LPS injection. (A) RPE/retina.

Prophase nuclear movements in wild-type, rec8 and rec7 mutant cells.

IGF1R is present in endometrial luminal epithelium at the receptive phase. IGF1R is present in endometrial luminal epithelium at the receptive phase. Endometrial.

Association of MMP expression with macrophages.

CENP-F staining in prometaphase and metaphase spermatocytes from B6 and Rb/+ mice. CENP-F staining in prometaphase and metaphase spermatocytes from B6.

Vessel density is decreased in tumors grown in Fbln5−/− mice.

Fig. 4. CR4.2 may be active in amacrine cells but not in ganglion cells.Chick retinas were electroporated with either the control CAG-GFP construct or.

Peroxisome speeds were slower in patient and control cells.

Histology of Brucella bone marrow foci in mice.

Oxidative stress is elevated in tumors grown in Fbln5−/− mice.

Adult Acvr1;Trp53DCKO lenses form vascularized, tumor-like structures at their posterior poles. 4-month-old Trp53CKO and Acvr1;Trp53DCKO lenses were sectioned.

Histone crotonylation in kidney tubular cells.

BMOL3 LPCs can differentiate towards the hepatocyte lineage.

Telomere cohesion is established in S phase in cohesin-ring-depleted cells. Telomere cohesion is established in S phase in cohesin-ring-depleted cells.

Disruption of the tubule formation by immortalized cells during nephrogenesis in vitro. Disruption of the tubule formation by immortalized cells during.

2DG suppresses of lamellipodia and filopodia and causes disorganization of F-actin filaments in murine endothelial cells. 2DG suppresses of lamellipodia.

Detection of phosphorylated Smad2/3 (pSmad2/3) in the skin of wild-type and transgenic animals during the first hair cycle. Detection of phosphorylated.

Upon challenge with oxazolone, human leukocytes (mainly consisting of T cells) infiltrate the dermis and epidermis. Upon challenge with oxazolone, human.

Β-catenin knockout inhibits synovial sarcoma (SS) tumor growth in SYT–SSX2 transgenic mice. β-catenin knockout inhibits synovial sarcoma (SS) tumor growth.

Presentation transcript:

Detection of nuclear foci by FISH on frozen sections of diaphragm muscle prepared from 2-month-old DMSXL mice. Detection of nuclear foci by FISH on frozen sections of diaphragm muscle prepared from 2-month-old DMSXL mice. Using the Cy3-labeled CAG-repeats PNA-probe, the RNA inclusions are labeled in red within the nuclei of muscle fibers stained in blue with DAPI. (A) Low magnification image shows numerous nuclei stained in blue surrounding the diaphragm muscle fibers. (B,C) At higher magnification the presence of one or more foci inside the nuclei is easily observed. Scale bars: 20 μm (A); 5 μm (B,C). Petrica-Adrian Panaite et al. Dis. Model. Mech. 2013;6:622-631 © 2013. Published by The Company of Biologists Ltd