Effects of cgh-1 RNAi on oogenesis and spermatogenesis.

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Effects of cgh-1 RNAi on oogenesis and spermatogenesis. Effects of cgh-1 RNAi on oogenesis and spermatogenesis. (A and B) Nomarski DIC views of gonads within 1-day-old wild type (A) and cgh-1(RNAi) (B) hermaphrodites. d, distal; l, loop; s, sperm; o, oocytes; do, degraded oocytes and e, embryo. (C-J) Expression of germline proteins in gonads extruded from 1-day-old wild type (C,E,G,I) and cgh-1(RNAi) (D,F,H,J) hermaphrodites. Gonads were stained with antibodies to the NOTCH homolog GLP-1 (Crittenden et al., 1994; C-D), the meiotic protein GLD-1 (Jones et al., 1996; E-F), the yolk receptor RME-2 (Grant and Hirsh, 1999; G-H), and the P granule component PGL-1 (I-J). d, distal; p, proximal. (K, L) Sperm that were prepared from wild-type and cgh-1(RNAi) gonads were induced with pronase to visualize their pseudopods. Boxes surround a detailed view. Rosa E. Navarro et al. Development 2001;128:3221-3232 © 2001.