Overexpression of individual signaling pathway components does not phenocopy the cell-spreading phenotype induced by elevated Hipk. Overexpression of individual.

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Overexpression of individual signaling pathway components does not phenocopy the cell-spreading phenotype induced by elevated Hipk. Overexpression of individual signaling pathway components does not phenocopy the cell-spreading phenotype induced by elevated Hipk. (A) A third instar wing imaginal disc with HA-hipk3M+GFP expressed along the dpp domain serves as the baseline phenotype/control disc. Individual pathway activators were expressed using dpp-Gal4, UAS-GFP, namely: (B) UAS-ArmS10, (C) UAS-Stat92E, (D) UAS-Rasact, (E) UAS-eiger, (F) constitutively active UAS-ykiS168A, (G) UAS-Nact and (H) UAS-Ci. Discs were stained for F-actin (magenta) to reveal tissue morphology and for GFP (green, white) to mark cells in which transgenes were ectopically expressed using dpp-Gal4. Scale bars: 50 μm. All crosses were done at 29°C. Jessica A. Blaquiere et al. Dis. Model. Mech. 2018;11:dmm031146 © 2018. Published by The Company of Biologists Ltd