Loss of serotonergic markers after Smed-pitx RNAi is not due to stem cell-based turnover. Loss of serotonergic markers after Smed-pitx RNAi is not due.

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Loss of serotonergic markers after Smed-pitx RNAi is not due to stem cell-based turnover. Loss of serotonergic markers after Smed-pitx RNAi is not due to stem cell-based turnover. WISH (A-E), FISH (F-J) and immunostainings (F′-J′) are shown. (A) Smed-sert expression in control animals. (B-E) Loss of Smed-sert expression after Smed-pitx RNAi from 1 to 5 dpi (days after the last of three consecutive injections). (F-J) Smed-sert and (F′-J′) SMEDWI-1 expression from 4 to 12 days post-γ-irradiation (dpirr). Note that serotonergic neurons are not affected throughout the timecourse, whereas SMEDWI-1+ stem cell progeny are entirely lost after irradiation (20/20 animals per time point), indicating a relatively slow turnover of serotonergic neurons. Scale bars: 500 μm in A-I′; 250 μm in J,J′. Martin März et al. Development 2013;140:4499-4509 © 2013. Published by The Company of Biologists Ltd