Neighbor-joining tree based on nifH for 20 organisms along with the maximum-likelihood sequence obtained from aligning the soil data to the gene sequence for nifH of B. valentinum LmjM3. Neighbor-joining tree based on nifH for 20 organisms along with the maximum-likelihood sequence obtained from aligning the soil data to the gene sequence for nifH of B. valentinum LmjM3. Bootstrap values are shown next to the branches, and the bar indicates 0.5 nucleotide substitutions per site. David Koslicki, and Daniel Falush mSystems 2016; doi:10.1128/mSystems.00020-16