Fig. 3. Reduced histone H3K9 trimethylation in SCNT embryos treated with TSA and VC. (A) Immunostaining analysis indicated that SCNT embryos with TSA+VC.

Slides:

Advertisements

Similar presentations

Scanning electron microscopy analysis of EGK-I to -V chick embryos.

Advertisements

RGFP-966 increases H4K12 acetylation in HEK/APPsw cells.

The SI-Induced Increase in PA Levels Is Mediated by PbrPLDδ1 in Pollen

Self-Incompatible Pollen Tubes Exhibit Increased PA Accumulation.

Fig. 2. Effect of endurance training on gene expression, and protein content and activity in heart muscle. Effect of endurance training on gene expression,

Fig. 3. Effect of NH4Cl (0 or 30 mM) on percentage of motile spermatozoa and VAP after 1 and 5 min after activation. Effect of NH4Cl (0 or 30 mM) on percentage.

Fig. 1. Chlamydia infection causes elevated levels of sortilin.

Fig. 2. Morphological changes of cultured adherent fibroblastic cells after OA treatment related to actin microfilament reorganization.(A) Cells observed.

Fig. 7. Motion adaptation increases time-dependent response modulations (TDRM) relatively to the average cell response.TDRM normalized to the value obtained.

Fig. 4. Seeding density modulates cell shape in both cell types

Fig. 2. Proportion of motile objects and track length

Fig. 4. Phosphorylation of the MARCKS-homology domain partially inhibits Hts' ability to disrupt Dlg postsynaptic targeting to the larval NMJ.(A–D″) High.

Fig. 4. A primary screen based on scrape closure

Fig. 3. Knockdown of cited3 results in increased cell death but it does not affect proliferation.Embryos that are injected with the control MO (A–C), cited3.

Fig. 6. Cross-section of the stomach wall and spiral intestine of the embryo, stained with PAS. (A) Surface of the stomach wall (SW) and ingested material.

Effects of embryonic hypoxia on fasted and postprandial cardiac shunting. Effects of embryonic hypoxia on fasted and postprandial cardiac shunting. Mean.

Fig. 4. Effects of ion deprivation on percentage of motile spermatozoa and VAP after 1 and 5 min after activation. Effects of ion deprivation on percentage.

Table 1. Menthol preference index (MPI) and average number of eggs laid per female (EPF) in F0 and F1 lines.The statistical significance of MPI was assessed.

Fig. 10. Ratiometric live imaging of di-4-ANEPPDHQ in growing pollen tubes.(A) Higher and lower membrane order distribution in control and BCD treated.

Fig. 1. Mitochondrial internalization in cardiomyocytes.

Fig. 3. Read-outs of mTORC1 (P-S6(S235/236)) and mTORC2 (P-Akt(S473)) in wtPC12 and PC12-27 cells.(A,B) wtPC12 and PC12-27 cells were treated for 48 hr.

Fig. 3. Inactivation of the Wnt/β-catenin signaling pathway inhibited cell proliferation and induced apoptosis in A549 and SPC-A-1 cells. Inactivation.

Fig. 4. The model of malate metabolism in fruit cells under different K level conditions. The model of malate metabolism in fruit cells under different.

Effects of embryonic hypoxia on fasted and postprandial cardiac output

Effects of embryonic hypoxia on fasted and postprandial heart rate and stroke volume. Effects of embryonic hypoxia on fasted and postprandial heart rate.

Fig. 1. IL-31 and IL-31R mRNA expression was upregulated in the asthma mouse model. IL-31 and IL-31R mRNA expression was upregulated in the asthma mouse.

Fig. 4. Brood size of four successive births by male seahorses Hippocampus erectus in the two groups (TR-1, TR-2).In TR-1 groups: male and female seahorses.

Fig. 5. Morphological changes of the N

Fig. 5. The bulk of Cep135 localizes distantly from Sas-6 and STIL

Fig. 6. Comparison of Plk4 with Sas-6 localization

Fig. 7. ML mediated suppression of SAHA-induced up-regulation of tRNA as a potential mechanism that contributes to the enhanced anti-cancer actions.

Fig. 6. Effect of SAHA and ML on histone acetylation, BAX, and p21CDKN1A expression.PANC-1 and BxPC-3 cells were incubated for 48 hours with 5 µM.

Fig. 7. Lhx1-RNAi reduces the eye size

Fig. 1. Effects on the tight junction barrier and permeability following doxorubicin and Herceptin treatment. Effects on the tight junction barrier and.

Fig. 2. Effect of substrate orientation on growth rate of midwater tadpoles with different oral configurations. Effect of substrate orientation on growth.

Fig. 2. Soluble sugar and organic acid levels with different K fertilization during fruit development. Soluble sugar and organic acid levels with different.

Fig. 1. Aboveground biomass of Caragana and herbaceous plants, and proportional abundance of Caragana, under different grazing management treatments. Aboveground.

Fig. 7. Representative images of control (Cas9+GFP) and Cas9+gRNA+GFP co-injected embryos on day 4 of culture, showing nuclear-imported GFP (green) and.

Fig. 1. TSA at 165 nM induces maximal acetylation of histone H3 and near-maximal acetylation of histone H4.Immunoblot analysis of acetylated histones H3.

Fig. 4. Co-immunostaining of nocodazole or ASNase treated RPE-1 cells with anti-hASNS and anti-alpha tubulin showed defect in both mitotic spindle formation.

Delayed formation of sensory hair cells in dlx3b/4b-deficient embryos

Fig. 3. Phospholipid fatty acids in N. nana brown adipose tissue.

The effect of TDP-43A315T expression on dendrite spine development.

Fig. 3. Effect of substrate orientation on growth rate for bottom-dwelling tadpoles with similar oral configuration. Effect of substrate orientation on.

Fig. 2. STK35 RNAs are expressed in distinct yet overlapping cell populations in adult mouse testis. STK35 RNAs are expressed in distinct yet overlapping.

Fig. 6. STK35 KO mice show ovary defects.

Fig. 5. Combination of SAHA and ML produces augmented suppression of cellular proliferation with impaired cell cycle progression, enhanced apoptotic.

Averages from seven separate experiments of primary myoblast cultures grown in HS-2; HS-2+GAL-M; HS-2+COS-1; FCS-20; FCS-20+GAL-M; or FCS-20+COS-1. Averages.

Fig. 2. TSA impairs growth and disrupts morphogenesis of exocrine pancreas in zebrafish larvae with hyperacetylation of nucleosomal histones.WT zebrafish.

Statistical chart of significantly differentially expressed genes

Fig. 4. Quantitative mRNA expression of two membrane-bound trehalase genes in Harmonia axyridis in response to starvation (0–72 h). Quantitative mRNA expression.

Effect of the plastid translation inhibitor Spec on LR development

Fig. 2. Immunostaining for ClC-5 in isolated hog gastric mucosa

Effect of γ-secretase inhibition on the vulnerability of primary neurons to OGD. (A) Experimental design. Effect of γ-secretase inhibition on the vulnerability.

Decreasing sleep levels using dTRPA1-based reversible activation of dopaminergic neurons reversibly decreases egg output. Decreasing sleep levels using.

The transcriptional activation activity of PIFs is higher in the cop1-4hfr1 background compared with the cop1-4 background. The transcriptional activation.

Fig. 2. iPSCs produce functional osteoblasts.

KIF13A overexpression favors NP distribution to the cell edge and requires KIF13A binding to microtubules. KIF13A overexpressionfavorsNP distribution to.

Fig. 2. Effects of pH on percentage of motile spermatozoa and VAP after 1 and 5 min after activation. Effects of pH on percentage of motile spermatozoa.

KIF13A depletion leads to a drop in virus titers, without affecting viral protein expression. KIF13A depletion leadsto a drop in virus titers, without.

Fig. 2. Acetylation stiffens primary cilia.

Fig. 5. Testis defects in STK35 KO mice.

The vps13 mutants are hypersensitive to SDS but cell wall integrity is not compromised. The vps13 mutants are hypersensitive to SDS but cell wall integrity.

Fig. 7. Eye defects in STK35 KO mouse.

Fig. 5. Co-expression analyses of disease mutations in YFP-RPGRIP1α1 with wild-type RFP-RPGR1–19 or RFP-RPGRORF15 in COS7 cells.YFP-RPGRIP1α1 with disease-associated.

Summary of variabilities in hiPSC-CMs.

Decreased enzymatic activity in PKG mutants increases time to recovery from an electroshock. Decreased enzymatic activity in PKG mutants increases time.

Effect of cocaine (COC) on the likelihood and rate of round dancing by bees returning from a 1.5 mol l–1 sucrose feeder. Effect of cocaine (COC) on the.

Effect of cocaine (COC) on sucrose responsiveness

Effect of cocaine on locomotion.

Presentation transcript:

Fig. 3. Reduced histone H3K9 trimethylation in SCNT embryos treated with TSA and VC. (A) Immunostaining analysis indicated that SCNT embryos with TSA+VC showed a significantly lower level of H3K9me3 compared to control embryos. Reduced histone H3K9 trimethylation in SCNT embryos treated with TSA and VC. (A) Immunostaining analysis indicated that SCNT embryos with TSA+VC showed a significantly lower level of H3K9me3 compared to control embryos. Different letters indicate statistical significance (P<0.05, one-way ANOVA with subsequent Tukey's multiple comparison tests). Fourteen or fifteen two-cell embryos were examined. Error bars represent means±s.e. (n=3). (B) A model shows how the treatment with trichostatin A and vitamin C in the dBSA-containing medium enhances epigenetic reprogramming and results in the enhanced cloning success rate. Kei Miyamoto et al. Biology Open 2017;6:415-424 © 2017. Published by The Company of Biologists Ltd