α-toxin and PVL-mediated cytotoxicity is blocked by IVIG

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α-toxin and PVL-mediated cytotoxicity is blocked by IVIG α-toxin and PVL-mediated cytotoxicity is blocked by IVIG. (A) Western blot analyses of α-toxin, rPVL and bacterial supernatants at indicated concentrations/dilutions. α-toxin and PVL-mediated cytotoxicity is blocked by IVIG. (A) Western blot analyses of α-toxin, rPVL and bacterial supernatants at indicated concentrations/dilutions. The blots were probed using IVIG as primary antibody followed by a secondary anti-human IgG antibody. (B,C) Lung epithelial cells (16HBE) (B) and primary human neutrophils (C) were exposed to bacterial supernatants from indicated strains or to toxins (α-toxin 500 ng/ml; rPVL 150 ng/ml) in the presence of different concentrations of IVIG. Cytotoxicity was assessed by lactate dehydrogenase (LDH) release assay. The bars show mean±s.d. of two individual experiments. (D) Intensity sum (GFP) in lung tissue models exposed for 16 h with 2.5 mg/ml IVIG alone, NP753, NP753+IVIG, supernatants from neutrophils (PMN) exposed to NP753, and supernatants from PMN stimulated with NP753+IVIG. Mean±s.d. of three individual experiments are shown. (E) Intensity sum (GFP) in lung tissue models exposed for 16 h to 2.5 mg/ml IVIG alone, LE2332, LE2332+IVIG, supernatants from PMN stimulated with LE2332, and supernatants from PMN exposed to LE2332+IVIG. Mean±s.d. of three individual experiments are shown. Statistical differences between presence or absence of IVIG were determined using the Mann–Whitney test; *P<0.05, **P<0.01. Srikanth Mairpady Shambat et al. Dis. Model. Mech. 2015;8:1413-1425 © 2015. Published by The Company of Biologists Ltd